SysmexXT-4000i全血细胞分析仪在体液细胞.PDFVIP

?·论 著· Sysmex XT-4000i 全血细胞分析仪在体液细胞 计数及分类中的应用 陈姣，黄茜，丁彬，毛志刚，粟军 四川大学华西医院实验医学科（成都? 610041） 【摘要】 ? 目的 ? ?评价 Sysmex XT-4000i 全血细胞分析仪体液模式在胸腹腔积液及脑脊液标本细胞计数及分 类计数中的应用价值。方法 ? ?采集 2015 年 5 月–9 月住院患者胸腹腔积液及脑脊液标本共 95 份，应用 Sysmex XT- 4000i 全血细胞分析仪（仪器法）和改良牛鲍计数板（手工法）分别计数，对两种方法计数结果进行比较。结果 ? ?手 工法与仪器法体液标本有核细胞计数及红细胞计数一致性较好（kappa=0.965，P0.001；kappa=0.988，P0.001）； 有核细胞分类计数中单个核细胞计数结果差异无统计学意义（P0.05），而多个核细胞计数结果差异有统计学意 义（P0.05）。结论 ? ?全血细胞分析仪体液模式精密度高，效率高，易于大批量操作，可能替代部分物理性状较好 的常规胸腹腹腔积液和脑脊液标本的细胞计数，但细胞分类计数结果还需要显微镜检查辅助。 【关键词】 ?全血细胞分析仪；胸腔积液；腹腔积液；脑脊液；细胞计数；细胞分类 Application of Sysmex XT-4000i Hematology Analyzer in the Cell Count and Cell Differential Count of Body-fluid Sample Chen Jiao, Huang Qian, Ding Bin, Mao Zhigang, Su Jun Department of Laboratory Medicine, West China Hospital/West China Medical School, Sichuan University, Chengdu, 610041, P.R.China Corresponding author: Su Jun, Email: sjhuaxi1667@163.com 【Abstract】 Purpose ? ?To evaluate the value of body-fluid model of Sysmex XT-4000i hematology analyzer in cell count and classification of pleural effusion, ascitic fluid and cerebrospinal fluid. Method ? ?A total of 95 samples, including pleural effusion, ascitic fluid and cerebrospinal fluid, were collected between May 1, 2015 to September 30, 2015. The samples were tested by Sysmex XT-4000i hematology analyzer (instrument method) and modified Neubauer hemocytometer (manual method). Result ? ?Instrument method and manual method have good consistency in nuclear cell count and erythrocyte count (Kappa=0.965, P0.001; Kappa=0.988, P0.001). There was no significant difference in the count of mononuclear cell counts (P0.05). However, its show significant difference in the count of multiple nuclear cell. Conclusions ? ?The body-fluid model of hematology analyzer has high precision, high efficiency and easy operation, which can instead of the manual method in the count of pleural effusion, ascitic fluid and cerebrospinal fluid in well physical properties, but the cell differential count also