DNARepairGenePolymorphismsand.PDFVIP

ANTICANCER RESEARCH 26: 4955-4958 (2006) DNA Repair Gene Polymorphisms and Bladder Cancer Susceptibility in a Turkish Population BENSU KARAHALIL1, NESLIHAN AYGüN KOCABAS1 and TAYFUN ?Z?ELIK2 1Gazi University, Faculty of Pharmacy, Department of Toxicology, Ankara; 2Bilkent University, Faculty of Science, Department of Molecular Biology and Genetics, Ankara, Turkey Abstract. Background: Occupational exposure and life style associated with mutations that activate oncogenes or preferences, such as smoking are the main known environmental inactivate tumor suppressor genes (4). Cigarette smoking is susceptibility factors for bladder cancer. A growing list of the dominant risk factor for several epithelial cancers, chemicals has been shown to induce oxidative DNA damage. including the lung, bladder, oral cavity, pharynx and larynx (5). Base excision repair (BER) genes (X-ray repair cross Smoking constitutes the single most important cause of complementing 1, XRCC1 and human 8-oxoguanine DNA bladder cancer, with cigarette smokers having 2- to 4-fold glycosylase 1, OGG1) may play a key role in maintaining genome higher incidences than nonsmokers, and causes direct and integrity and preventing cancer development. Materials and indirect damage to DNA (6, 7). The repair of DNA damage is Methods: We tested whether polymorphisms in XRCC1 and under genetic control. DNA repair genes may play a key role OGG1 are associated with bladder cancer risk by using in maintaining genome integrity and preventing cancer Polymerase Chain Reaction-Restriction Fragment Length development. At least 135 single nucleotide polymorphism Polymorphism (PCR-RFLP) assay. In addition, the possible (SNPs) in 17 DNA repair and repair related genes have been modifying affect of cigarette smoking was evaluated. Results: No identified, including genes for nucleotide excision repair studies, to date, have examined the assoc