《基因工程》-选择题.docVIP

1. Prokaryotes and eukaryotes use several methods to regulate gene expression, but the most common method is ( )? A. translational control B. transcriptional control? C. posttranscriptional control D. control of mRNA passage from the nucleus 2. What effect would the addition of lactose have on a repressed lac operon? ( )??? ?A. The operator site on the operon would move B. It would reinforce the repression of that gene?C. The lac operon would be transcribed???? D. It would have no effect whatsoever 3. Cutting certain genes out of molecules of DNA requires the use of special ( ) A.degrading nucleases B. restriction endonucleases C. eukaryotic enzymes D. viral enzymes4. A DNA library is ( ) ???A. a general collection of all genes sequenced thus far. B. a collection of DNA fragments that make up the entire genome of a particular organism. C. a DNA fragment inserted into a vector. D. all DNA fragments identified with a probe. 5. A probe is used in which stage of genetic engineering? ( )???? A cleaving DNA B. recombining DNA C. cloning D. screening 6. The enzyme used in the polymerase chain reaction is ( ) A. restriction endonuclease B. reverse transcriptase?? ?C. DNA polymerase D. RNA polymerase7.下列对DNA聚合酶的描述哪一种是错误的( ) A. 具有5`-3`外切活性 B. 具有5`-3`聚合活性 C. 具有3`-5`外切活性 D. 具有3`-5`聚合活性 8. 大肠杆菌mRNA上起始密码子上游的SD序列可与某种RNA的3`端配对,然后启动多肽链合成，这种RNA是（ ） A . tRNA B. SnRNA C. 16sRNA D. 23s rRNA 9.反转录酶是一种（ ） A. 依赖DNA的DNA聚合酶 B. 依赖DNA的RNA聚合酶 C. 依赖RNA的DNA聚合酶 D. 依赖RNA的DNA聚合酶 10. 根据基因工程的定义，下列各名词中不能替代基因工程的是( )A. 基因诱变 B. 分子克隆 C. DNA重组 D. 基因无性繁殖 11.下列五个 DNA 片段中含有回文结构的是( )A. GAAACTGCTTTGAC B. GAAACTGGAAACTG C. GAAACTGGTCAAAG D. GAAACTGCAGTTTC 12.T4 -DNA 连接酶是通过形成磷酸二酯键将两段 DNA 片段连接在一起，其底物的关键基团是 ( )A. 2 -OH 和 5 -P B. 2 -OH 和 3 -P C. 3 -OH 和