Biosensors and Bioelectronics-Efficient one-pot synthesis of molecularly imprinted silica.pdf

Biosensors and Bioelectronics 38 (2012) 55–60Contents lists available at SciVerse ScienceDirectBiosensors and Bioelectronics0956-56 http://d n Corr E-mjournal homepage: /locate/biosEfficient one-pot synthesis of molecularly imprinted silica nanospheres embedded carbon dots for fluorescent dopamine optosensingYan Mao, Yu Bao, Dongxue Han n, Fenghua Li, Li Niu n State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Graduate University of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun 130022, PR Chinaa r t i c l e i n f o Article history: Received 2 March 2012 Received in revised form 25 April 2012 Accepted 29 April 2012 Available online 10 May 2012 Keywords: Molecularly imprinted polymer Sol–gel polymerization Carbon dots Fluorescence sensor Dopamine63/$ - see front matter 2012 Elsevier B.V. A /10.1016/j.bios.2012.04.043 esponding authors. Tel.: t86 431 ail addresses: dxhan@ (D. Han), lniu@a b s t r a c t A new type of eco-friendly molecularly imprinted polymer (MIP) was synthesized through an efficient one-pot room-temperature sol–gel polymerization and applied as a molecular recognition element to construct dopamine (DA) fluorescence (FL) optosensor. Highly luminescent carbon dots (CDs) were firstly synthesized via a one-step reaction in organosilane, and their surface were anchored with MIP matrix (CDs@MIP). The resulting composite of a synergetic combination of CDs with MIP showed high photostability and template selectivity. Moreover, the composite allowed a highly sensitive determination of DA via FL intensity decreasing when removal of the original templates. The new MIP-based DA sensing protocol was applied to detect DA concentration in aqueous solution, the relative FL intensity of CDs@MIP decreased linearly with the increasing DA in the concentration range of 25–500 nM with a detection limit (3s) of 1.7 nM. Furthermore, th