- 1、本文档共7页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
Callus induction and regeneration of elite Indian maize
African Journal of Biotechnology Vol. 9(44), pp. 7446-7452, 1 November, 2010
Available online at /AJB
ISSN 1684–5315 ?2010 Academic Journals
Full Length Research Paper
Callus induction and regeneration of elite Indian maize
inbreds
A. Manivannan*, J. Kaul, A. Singode and S. Dass
Directorate of Maize Research, Pusa Campus, New Delhi-110012, India.
Accepted 27 September, 2010
Five elite Indian maize inbreds namely; HKI1105, HKI1105, HKI335, CM300 and LM5 were evaluated for
callus induction and regeneration. Immature embryos obtained 14 days after pollination were used as
explants. Genotype, medium, type of auxin and their concentrations influenced callus induction. N6
medium supplemented with different concentration of 2,4-D (1, 2 and 3 mg/l) and Dicamba (1, 2 and 3
mg/l) were used for callus induction. N6 supplemented with 2 mg/l of 2,4-D has shown highest
percentage of embryogenic callus induction. Among the five genotypes tested, CM300 gave highest
percentage of embryogenic calli. CM300 and LM5 both have shown higher regeneration percentage of
12.22%.
Key words: Maize, in-vitro culture and regeneration.
INTRODUCTION
Maize (Zea mays L.) is the most important cereal crop in
the world in terms of global annual tons produced (Food
and Agricultural Organization, 2009). Maize is raw
material for a number of industrial products besides its
uses as human food and animal feed. At present, the
developed world uses maize more than the developing
world, but forecasts indicate that by the year 2020, the
developing countries will demand more maize than the
developed world (Duvick, 1998). One of the strategies to
mitigate various stresses in maize is development of
transgenic maize. Genetic transformation of maize with
genes conferring resistance to biotic/abiotic stresses is
expected to address many of these issues synergistically
with conventional breeding.
Green and Philips (1975) first reported regeneration of
您可能关注的文档
- Barack Obama and the Bush Tax Cut (A).pdf
- Barrel Muon System Background Simulation of the CMS TDR Design.pdf
- BackTrack_基础安装介绍.pdf
- BAN_07_HFC_2008.pdf
- Bao-2011-An immune-induced Re.pdf
- Bang_2011_Landscape-and-Urban-Planning.pdf
- BASF树脂_finishing.pdf
- BARRY SMITH.pdf
- Bauxite ‘red mud’ in the ceramic industry Part 1thermal behaviour.pdf
- Barron阅读文本第一套.pdf
- 10《那一年,面包飘香》教案.docx
- 13 花钟 教学设计-2023-2024学年三年级下册语文统编版.docx
- 2024-2025学年中职学校心理健康教育与霸凌预防的设计.docx
- 2024-2025学年中职生反思与行动的反霸凌教学设计.docx
- 2023-2024学年人教版小学数学一年级上册5.docx
- 4.1.1 线段、射线、直线 教学设计 2024-2025学年北师大版七年级数学上册.docx
- 川教版(2024)三年级上册 2.2在线导航选路线 教案.docx
- Unit 8 Dolls (教学设计)-2024-2025学年译林版(三起)英语四年级上册.docx
- 高一上学期体育与健康人教版 “贪吃蛇”耐久跑 教案.docx
- 第1课时 亿以内数的认识(教学设计)-2024-2025学年四年级上册数学人教版.docx
文档评论(0)