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identification and fine mapping of rhm1 locus for resistance to Southern Corn Leaf Blight in Maize.pdf

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identification and fine mapping of rhm1 locus for resistance to Southern Corn Leaf Blight in Maize

Journal of Integrative Plant Biology 2012, 54 (5): 321–329 Research Article Identification and Fine Mapping of rhm1 Locus for Resistance to Southern Corn Leaf Blight in MaizeF Yuanzeng Zhao1,2?, Xiaomin Lu1,3?, Chaoxian Liu1, Haiying Guan1, Mei Zhang1, Zhongfeng Li1, Hongwei Cai1 ? and Jinsheng Lai1 ? 1State Key Laboratory of Agrobiotechnology and National Maize Improvement Center, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China 2Department of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China 3Henan Academy of Agricultural Sciences, Zhengzhou 450002, China ?These two authors contributed equally to this study. ? Corresponding authors Tel: +86 10 6273 1405; Fax: +86 10 6273 1416; E-mail: caihw@; jlai@ F Articles can be viewed online without a subscription. Available online on 21 February 2012 at and /journal/jipb doi: 10.1111/j.1744-7909.2012.01112.x Abstract rhm1 is a major recessive disease resistance locus for Southern corn leaf blight (SCLB). To further narrow down its genetic position, F2 population and BC1F1 population derived from the cross between resistant (H95rhm) and susceptible parents (H95) of maize (Zea mays) were constructed. Using newly developed markers, rhm1 was initially delimited within an interval of 2.5 Mb, and then finally mapped to a 8.56 kb interval between InDel marker IDP961–503 and simple sequence repeat (SSR) marker A194149–1. Three polymorphic markers IDP961–504, IDP B2–3 and A194149–2 were shown to be co-segregated with the rhm1 locus. Sequence analysis of the 8.56 kb DNA fragment revealed that it contained only one putative gene with a predicted amino acid sequence identical to lysine histidine transporter 1 (LHT1). Comparative sequence analysis indicated that the LHT1 in H95rhm harbors a 354 bp insertion in its third exon as compared with that of susceptible alleles in B73, H95 and Mo17. The 354 bp insertion resulted in a truncation of the pre