J Appl Poult Res-2009-Applegate-552-61.pdfVIP

? 2009 Poultry Science Association, Inc. 2009 J. Appl. Poult. Res. 18 :552–561 doi: 10.3382/japr.2009-00010 Effect of amino acid formulation and dietary direct-fed microbial supplementation on egg production and egg characteristics in laying hens T. J. Applegate ,*1 E. M. Onyango ,? R. Angel ,? and W. J. Powers § * Department of Animal Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907-2054; ? Department of Health Sciences, East Tennessee State University, Johnson City 37614; ? Department of Animal and Avian Sciences, University of Maryland, College Park 20742; and § Department of Animal Sciences, Michigan State University, East Lansing 48824-1225 Primary Audience: Nutritionists, Researchers, Veterinarians, Flock Supervisors SUMMARY An experiment was conducted to determine whether direct-fed microbial supplementation could alleviate a marginal amino acid (AA) deficiency in Hyline 36 laying hens from 33 to 44 wk of age. The experiment was a 2 × 4 factorial design with or without a commercial direct- fed microbial (Primilac; 1.36 kg/1,000 kg) and 4 levels of AA formulation. Egg characteristics (yolk, albumen, or shell proportions and yolk or albumen solids) were not affected by diet. Primilac supplementation had no effect on egg production or egg mass. However, Primilac supplementation reduced feed intake-to-egg mass ratio by 2.4 and 3.4% from 33 to 36 wk and 41 to 44 wk, respectively. Total eggs laid and egg mass were greatest when at least 14.4 g of CP, 804 mg of Lys, 382 mg of Met, 601 mg of TSAA, 502 mg of Thr, and 609 mg of Ile were consumed per hen per day from 33 to 44 wk of age. In conclusion, Primilac supplementation was not able to completely alleviate a marginal AA deficiency in laying hens but did improve feed intake-to-egg mass ratios during 8 wk of the 12-wk study. Key words: amino acid , direct-fed microbial , egg production , laying hen DESCRIPTION OF PROBLEM Several reports have