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Leaf rust resistance gene Lr1, isolated from bread wheat.pdf

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Leaf rust resistance gene Lr1, isolated from bread wheat

Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family Sylvie Cloutier ? Brent D. McCallum ? Caroline Loutre ? Travis W. Banks ? Thomas Wicker ? Catherine Feuillet ? Beat Keller ? Mark C. Jordan Received: 14 February 2007 / Accepted: 8 June 2007 / Published online: 5 July 2007 Springer Science+Business Media B.V. 2007 Abstract In hexaploid wheat, leaf rust resistance gene Lr1 is located at the distal end of the long arm of chro- mosome 5D. To clone this gene, an F1-derived doubled haploid population and a recombinant inbred line popula- tion from a cross between the susceptible cultivar AC Karma and the resistant line 87E03-S2B1 were phenotyped for resistance to Puccinia triticina race 1-1 BBB that car- ries the avirulence gene Avr1. A high-resolution genetic map of the Lr1 locus was constructed using microsatellite, resistance gene analog (RGA), BAC end (BE), and low pass (LP) markers. A physical map of the locus was con- structed by screening a hexaploid wheat BAC library from cultivar Glenlea that is known to have Lr1. The locus comprised three RGAs from a gene family related to RFLP marker Xpsr567. Markers specific to each paralog were developed. Lr1 segregated with RGA567-5 while rec- ombinants were observed for the other two RGAs. Trans- formation of the susceptible cultivar Fielder with RGA567- 5 demonstrated that it corresponds to the Lr1 resistance gene. In addition, the candidate gene was also confirmed by virus-induced gene silencing. Twenty T1 lines from resis- tant transgenic line T0-938 segregated for resistance, partial resistance and susceptibility to Avr1 corresponding to a 1:2:1 ratio for a single hemizygous insertion. Transgene presence and expression correlated with the phenotype. The resistance phenotype expressed by Lr1 seemed there- fore to be dependant on the zygosity status. T3-938 sister lines with and without the transgene were further tested with 16 virulent and avirul