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Map-based cloning of the rice cold tolerance gene Ctb1.pdf

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Map-based cloning of the rice cold tolerance gene Ctb1

MK N a A R R A A K C Q F A R 1 c r O i t m t [ l a r a t s m e [ c i t 0 dPlant Science 179 (2010) 97–102 Contents lists available at ScienceDirect Plant Science journa l homepage: www.e lsev ier .com/ locate /p lantsc i ap-based cloning of the rice cold tolerance gene Ctb1 oji Saito ?, Yuriko Hayano-Saito, Makoto Kuroki, Yutaka Sato ational Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira, Sapporo, 062-8555, Japan r t i c l e i n f o rticle history: eceived 5 November 2009 eceived in revised form 9 April 2010 ccepted 9 April 2010 vailable online 23 April 2010 a b s t r a c t Low temperatures during the booting stage reduce rice yields by causing cold-induced male sterility. We previously mapped a quantitative trait locus for cold tolerance, Ctb1, to a 56-kb region containing 7 putative genes. In this studywe furthermapped Ctb1 to a 17-kb region containing two genes that encode an F-box protein and a ser/thr protein kinase. The F-box protein gene was preferentially expressed in young panicles, while the ser/thr protein kinase gene was expressed in leaves and young panicles. Botheywords: old tolerance TL -box protein nther length ice genes were cloned from a cold-tolerant variety, Norin-PL8, and introduced into a cold-sensitive variety, Hokkai241, and a cold-sensitive line, BT4-74-8. The cold tolerance of T2 and T3 progenies was assessed by measuring the degree of spikelet fertility in plants treated with cool water irrigation (19 ?C, 25 cm) or cool air (12 ?C, 4 days). The results indicated that the F-box protein gene confers cold tolerance. Cold tolerance is associated with greater anther length, and the transgenic plants had longer anthers than non-transformed controls. The F-box protein interacts with a subunit of the E3 ubiquitin ligase, Skp1, tin–psuggesting that an ubiqui . Introduction Rice is a staple crop cultivated worldwide. However, since it is a old-sensitive plant that evolved in tropical and subtropical areas, ice yields are