十六烷基三甲基溴化铵双层置换金纳米棒的硫醇交联剂增强等离子体共振灵敏度探究.pdfVIP

Replacement of Cetyltrimethylammoniumbromide Bilayer on Gold Nanorod by Alkanethiol Crosslinker for Enhanced Plasmon Resonance Sensitivity Justin Casas, Meenakshi Venkataramasubramani, Yanyan Wang, and Liang Tang* Department of Biomedical Engineering, University of Texas at San Antonio, San Antonio, TX 78249, U.S.A Abstract Surface modification of gold nanorods (GNRs) is often problematic due to tightly packed cetyltrimethylammoniumbromide (CTAB) bilayer. Herein, we performed a double phase transfer ligand exchange to achieve displacement of CTAB on nanorods. During the removal, 11- mercaptoundecanoic acid (MUDA) crosslinker is simultaneously assembled on nanorod surfaces to prevent aggregation. The resulting MUDA-GNRs retain the shape and position of plasmon peaks similar to CTAB-capped GNRs. The introduction of carboxyl groups allows covalent conjugation of biological receptors in a facile fashion to construct a robust, label-free biosensor based on localized surface plasmon resonance (LSPR) transduction of biomolecular interaction. More importantly, smaller MUDA layer on the GNRs reduces the distance of target binding to the plasmonic nanostructure interface, leading to a significant enhancement in LSPR assay sensitivity and specificity. Compared to modification using conventional electropolymer adsorption, MUDA- coated gold nanosensor exhibits five times lower detection limit for cardiac troponin I assay with a high selectivity. Keywords Gold nanorod; Surface modification; Functionalization; Biosensing; Surface plasmon resonance 1. Introduction In sensor applications, the optical transduction by gold nanoparticles is based upon the phenomenon of localized surface plasmon resonance, i.e. LSPR (Lee and El-Sayed 2006). According to the plasmon hybridization mode (Prodan et al. 2003), the plasmon resonance frequency is largely determined by the geometry of the colloidal metal structure, as a result of electrostatic interactions between confined electrons distributed