场流分级生物分析：近期的发展趋势.pdfVIP

Analytica Chimica Acta 635 (2009) 132–143 Contents lists available at ScienceDirect Analytica Chimica Acta journal homepage: /locate/aca Review Field-?ow fractionation in bioanalysis: A review of recent trends Barbara Roda a,d, Andrea Zattoni a,d, Pierluigi Reschiglian a,d, Myeong Hee Moon b, Mara Mirasoli c,d, Elisa Michelini c,d, Aldo Roda c,d,? a Department of Chemistry “G. Ciamician”, University of Bologna, Via Selmi 2, 40126 Bologna, Italy b Department of Chemistry, Yonsei University, Seoul 120-749, South Korea c Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy d I.N.B.B. Interuniversity Consortium, Rome, Italy article info abstract Article history: Field-?ow fractionation (FFF) is a mature technique in bioanalysis, and the number of applications to Received 13 October 2008 proteins and protein complexes, viruses, derivatized nano- and micronsized beads, sub-cellular units, Received in revised form 8 January 2009 and whole cell separation is constantly increasing. This can be ascribed to the non-invasivity of FFF when Accepted 9 January 2009 directly applied to biosamples. FFF is carried out in an open-channel structure by a ?ow stream of a mobile Available online 18 January 2009 phase of any composition, and it is solely based on the interaction of the analytes with a perpendicularly applied ?eld. For these reasons, fractionation is developed without surface interaction of the analyte Keywords: