已改 陶卓玺.docVIP

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已改 陶卓玺.doc

For oomycetes on wild host plants, this phenomenon has not been explored yet, whereas seed contamination of crop plants was shown for the downy mildew pathogen of basil (Belbahri et al. 2005) as well as for the white blister rust Pustula on sunflower (Viljoen et al. 1999). With the globalization of seed trading, this way of pathogen distribution has become one of the major problems in agri- and horticulture. Quarantine regulations try to impede seed transmission of diseases, but fast, sensitive and reliable detection methods are still mostly lacking. While germinating and cultivating an appropriate amount of seeds, evaluating the plants for disease symptoms is still the common way of testing seed contamination; molecular techniques have slowly been developed for oomycetes, but tests have not yet been brought into practical usage.For example, sunflower seed contamination with P. halstedii was investigated with various methods. PCR-based detection was attempted with selective oligonucleotide primers (Roeckel-Drevet et al. 1999; Says-Lesage et al. 2000) and with LSU-based primers (Ioos et al. 2007). An ELISA test was developed by Bouterige et al. (2000) and pathogen-specific fatty acids were used for the detection by Spring and Haas(2004). In Peronospora parasitic to basil, specific primers deduced from ITS sequences not only allowed the detection of the downy mildew of basil in seeds and plant tissue, but also enabled quantification of the contamination by using real-time PCR (Belbahri et al. 2005). However, in many cases, the problem for bringing such a test into practice is not only the sensitivity of system, which, in case of sunflower, has proven to be close tomarket needs and allowed the detection of one contaminated seed out of 50 (Spring and Haas 2004) or even out of 400 (Thines et al. 2004). Perhaps the major obstacle is drawing of a representative sample from large seed batches. 对于野生寄生植物上有卵菌现象还没有被发现,然而作物的种子污染已经解释为霜霉病所为,同样的还有向日葵上的白疱锈病。随着种子的全球迁徙,这种病菌分布的方法

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