CHGangan生化課件酶.pptVIP

优秀精品课件文档资料;酶 --- Enzyme;教学大纲目的要求;Structure of Enzyme;Definition of Enzyme;Components of Enzyme;;Some terms should be noticed;Characteristics of enzymatic reaction;Mechanism of catalytic reaction;Some terms ; Kinetics of enzymatic reaction;;米－曼氏方程式推导基于两个假设： E与S形成ES复合物的反应是快速平衡反应，反应速度取决于慢反应ES分解为E及P。即 V＝k3[ES] (1) S的总浓度远远大于E的总浓度，在反应的初始阶段，S的浓度可认为不变。 即 [S]＝[St] ;稳态：是指ES的生成速度与分解速度相等，即 [ES]恒定。 K1 ([Et]－[ES]) [S]＝K2 [ES] + K3 [ES];[ES]＝─── ;当底物浓度很高，将酶的活性中心全部饱和时，即[Et]＝[ES]，反应达最大速度 Vmax＝K3[ES]＝K3[Et] (5);[S] influences the rate of reaction;Km and Vmax determination;Kinetics of enzymatic reaction;Kinetics of enzymatic reaction;Kinetics of enzymatic reaction;Kinetics of enzymatic reaction;Reversible inhibition;Competitive inhibition;Characteristic of competitive inhibition;双倒数动力学方程： 1 Km 〔I〕 1 1 V Vmax Ki 〔S〕 Vmax 双倒数作图： Vmax不变 表观Km增加 ;Case of competitive inhibition;磺胺类药物和对氨基苯甲酸竞争二氢叶酸合成酶;Reversible inhibition;Non-competitive inhibition;Characteristic of non-competitive inhibition;双倒数动力学方程： 1 Km 〔I〕 1 1 〔I〕 V Vmax Ki 〔S〕 Vmax Ki ;Reversible inhibition;Uncompetitive inhibition;Characteristic of uncompetitive inhibition;双倒数动力学方程： 1 Km 1 1 〔I〕 V Vmax 〔S〕 Vmax Ki ;作用特征 无抑制剂 CI Non-CI UnCI ;Influences of activators on the activity;Nomenclature of enzymes;Classification of enzyme ;The activity of enzyme is measured in IU;Enzyme and medicine;Enzyme and medicine;Enzyme and medicine;Enzyme and medicine;Enzyme and medicine;Enzyme and medicine