G-CSF对兔下腔静脉球囊损伤后内皮修复的影响.doc

基金名称：河南省教育厅科技攻关计划资助项目（2011A320025） 作者单位：450052 郑州，郑州大学第一附属医院普外科 通信作者：党晓卫，Email：dangxw1001@163.com G-CSF对兔下腔静脉球囊损伤后内皮修复的影响 党晓卫 王兆阳 乔师师 姚振涛 李林 王亚飞 许培钦 【摘要】 目的 观察粒细胞集落刺激因子（G-CSF）对兔下腔静脉球囊损伤修复中血管内皮变化的影响。方法 50只实验兔随机分为2组（n=25）：A组损伤后即刻皮下注射G-CSF 50μg/kg，连续7d；B组损伤后即刻皮下注射生理盐水（N-S）2ml，连续7d。两组均于术后7d、30d取损伤血管段，HE染色观察血管损伤及修复情况，计算内膜（I）、中膜（M）面积比值I/M 以评价内膜增生程度。结果 第7d，A组（0.0564±0.0303）与B组（0.0808±0.0428）组间差异无统计学意义（P=0.157）。第30d，A组（0.1462±0.0993）与B组（0.3098±0.2154）组间差异有统计学意义（P=0.043）。病理结果观察：两组???囊损伤后即刻均可见静脉内皮损伤。术后7天新生内膜已经形成并增厚，中膜可见梭形平滑肌细胞排列紊乱。术后30d A组内膜增生明显受到抑制。结论 G-CSF可促进兔下腔静脉球囊损伤修复的再内皮化，抑制内膜增生，可预防下腔静脉球囊损伤后再狭窄的发生。 【关键词】布-加综合征；球囊；内皮损伤；粒细胞集落刺激因子 Influence of Granulocyte colony stimulating factor to the rabbit inferior vena cava endothelial repair after balloon injury WANG Zhao-yang, DANG Xiao-wei , QIAO Shi-shi, YAO Zhen-tao, LI Lin, WANG YA Fei, XU Pei-qin. Department of General Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China Corresponding auth：DANG xiao-wei，Email：dangxw1001@163.com 【abstract】Objective To research the affect of rabbit inferior vena cava vascular endothelial repair after balloon injury by granulocyte colony- stimulating factor (G-CSF). Methods 50 experimental rabbit randomly divided into 2 groups（n=25）,Group A: rabbits were injected daily with 50 μg /kg G-CSF for 7 days shortly after inferior vena cava(IVC) injury. Group B: rabbits were injected daily with 2ml N-S for 7 days shortly after IVC injury. The animals were killed at 7 and 30 days after balloon denudation.The samples of IVC were harvested for observation damaged vascular repair situation by HE dyeing,count the intima(I) area and membrane(M) area ratio to evaluate intima hyperplasia degree. Results The 7 days of A( 0.0564±0.0303)and B group(0.0808±0.0428) I/M ratio comparison, difference was not statistically significant(P=0.157). The 30 days of A(0.1462±0.0993) and B group(0.3098±0.2154) I/M ratio comparison, difference have statistical significance(P=0.043). Path