多重评价液氮冷冻加被动强化运动建立股骨头坏死模型.pdf

·412 · CHINESE JOURNAL OF BONE AND JOINT INJURY Vol. 24 NO. 5. May. 2009 ·实验研究· 多重评价液氮冷冻加被动强化运动建立股骨头 坏死模型△ 童培建* 沈彦** 何帮剑** 金红婷** 赵红昌** 肖鲁伟** 摘要 目的 多重评价液氮冷冻加被动强化运动法建立股骨头坏死模型的效果。 方法 选用毕格犬 8 只，用液氮冷冻加术后被动 强化运动法制备股骨头坏死模型（实验侧），对侧对照（对照侧）。 术后 1、4、8、16 周取股骨头标本行苏木精－伊红染色；术后 1、8、16 周行 MRI 扫描；术前及处死前行数字减影血管造影术(DSA)。 结果 实验侧均出现不同程度股骨头坏死表现，且术后 16 周标本呈轻 度塌陷 示术后 周实验侧 出现高信号 内部散在低信号影 术后 周低信号影进一步扩大 示术后 周股骨头各 ；MRI 8 T2WI ， ， 16 ；DSA 16 支血管呈现不同的程度的狭窄或堵塞，并可见新生血管经股骨颈、髓腔等方向进入股骨头，但大多未能到达坏死区域。 结论 液氮 冷冻加被动强化运动法建立的股骨头坏死模型符合股骨头坏死的病理变化，是一种比较理想的造模方法。 关键词 股骨头坏死；动物模型；数字减影血管造影 Multiple Systems for Evaluatation of Models of Osteonecrosis of Femoral Head by Liquid Nitrogen Frozen Plus Passive Forced Motion Tong Peijian，Shen Yan，He Bangjian，et al. The First Hospital A ffiliated to Zhejiang University of TCM，Hangzhou，310006 Abstract Objective To evaluate an animal model of osteonecrosis of femoral head. Methods Eight Beagle dogs were selected，and the model of osteonecrosis of femoral head was established by liquid nitrogen frozen plus passive forced motion.Meanwhile，the left side served as control groups.Histological slices of femoral head were harvested on postoperative weeks 1、4、8 and 16 for HE staining.The osteonecro- sis of femoral head was observed by magnetic resonance imaging at 1、8 and 16 weeks after operation.DSA was performed before operation and sacrificing the animals to compare the pre-and post-operation blood supply surrounding femoral head. Results Path