河南科技大学万晶琼毕业论文.doc

4  PAGE 21 河 南 科 技 大 学 毕 业 设 计（论 文） 硝普钠对NaCl胁迫下黄瓜幼苗的影响 姓 名 万晶琼 院 （系） 林学院 专 业 园 艺 指导教师 蒋 燕 2014年 5月 15日 硝普钠对NaCl胁迫下黄瓜的影响 摘 要 一氧化氮（nitric oxide，NO）是生物体内的重要活性分子，在植物体内NO是一种广泛存在的信号分子，参与植物生长发育的许多重要的生理过程。本研究通过对根际施加外源NO供体硝普钠（sodium nitroprusside,SNP）,初步探讨了盐胁迫下NO处理对黄瓜幼苗生理生化的影响，为提高植物的抗盐性提供一定的理论依据。 在4g/Kg的NaCl的胁迫下，采用基质栽培研究SNP对黄瓜幼苗生长的影响，结果表明，盐胁迫抑制黄瓜的生长，而NO处理减弱了这种抑制作用，降低了盐胁迫下黄瓜叶片中可溶性蛋白质的含量，提高了抗氧化酶GPX，CAT的活性，说明低浓度NO提高了黄瓜叶片的抗氧化能力，减轻了盐胁迫诱导的膜质过氧化损伤，NO可能参与了植物的代谢或生理活动。盐胁迫显著降低了叶片叶绿素含量 ，显著增加了可溶性糖含量，低浓度的NO处理增加叶片叶绿素含量，降低了可溶性糖含量，说明NO有利于黄瓜非光能的捕获和转化，明显促进植物的生长，降低盐胁迫对黄瓜的抑制作用。另外，低浓度的SNP可以缓解盐胁迫对黄瓜幼苗的形态指标如株高，茎粗，叶长叶宽的抑制作用。 关键词：黄瓜，一氧化氮，低浓度，盐胁迫，SNP EFFECTS OF SODIUM SNP ON SEEDINGS OF CUCUMBER UNDER NaCl STRESS ABSTRACT Nitric oxide (NO) is an important active molecule in biology. It is now being evident that NO is also a ubiquitous signal molecule in plant kingdom. It was proved to participate in many key physiological processes.Therefore, the effects of NO donor on the physiological and biochemical mechanisms in hydroponic cucumber seedlings under salt stress were investigated, in order to clarify the possible mechanisms of enhancing salt-tolerance in plants. Under the stress of 4g/Kg NaCl, the impact of SNP on the growth of cucumber seedlings were studied by substrate culture. The results showed that salt stress can restrain the growth of the cucumber under salinity stress, and reduce the content of soluble protein in cucumber leaves under salt stress ,improve the antioxidant enzymes GPX, the activity of CAT. It could be concluded that low concentration NO improves the oxidation resistance of cucumber leaf, reduced the membranous peroxidation damage induced by salt stress, NO may be involved in the metabolism, or physiological activity of plants. Salt stress significantly decreased leaf chlorophyll content, significantly increased the soluble sugar content, low con