肝癌细胞受照后代生长特性及放射敏感性变化.docVIP

 HYPERLINK /2007/0305/D251FFFFC949T125610.shtml 肝癌细胞受照后代生长特性及放射敏感性变化 中华放射肿瘤学杂志 2000年第2期第9卷 论　著作者：石卫民　陈龙华单位：广州，第一军医大学南方医院放射治疗中心　510515关键词：HepG2细胞系；放射敏感性；p53　　【摘要】　目的　 探讨肝癌细胞照射后存活后代生长特性及放射敏感性的变化。 方法　人肝癌细胞株HepG2和其照射后存活后代体外培养，测定其群体倍增时间、放射敏感性和p53的表达水平。结果　HepG2的群体倍增时间为（96.0±6.8）h,克隆形成率为（13.0±1.5）%，SF2为0.41±0.05。HepG2照射后存活后代群体倍增时间为（124.8 ±12.2）h（t=3.572, P＜0.05），克隆形成率为（5.0±0.6）%（t=8.537, P＜0.01），SF2为0.65±0.08（t=3.811, P＜0.05），p53表达水平无差别（t=0.778, P＞0.05）。结论　 肝癌细胞照射后存活后代生长延缓，放射敏感性降低，但与p53的表达无关。 Radiosensitivity and growth characteristics of filial generation from irradiated hepatocarcinoma cells　　SHI Weimin　CHEN Longhua　　（Department of Radiation Oncology, Nanfang Hospital,First Military Medical University, Guangzhou 510515,China）　　【Abstract】　Objective　To investigate the change of radiosensitivity and growth features of the surviving progeny from the irradiated hepatocarcinoma cells. 　Methods　To cultured human hepatocarcinoma cells—HepG2 and the progeny of irradiated HepG2, plating efficiency(PE), population doubling time(PDT),radiosensitivity index SF2 and the expression level of p53 were measured. 　Results　The PDT of HepG2 was (96.0±6.8)hrs,PE (13.0±1.5)%，SF2 0.43±0.06.The PDT of the filial cells was (124.8 ±12.2)hrs（t=3.572, P＜0.05），PE (5.0±0.6)%（t=8.537, P＜0.01），SF2 0.65±0.08（t=3.811, P＜0.05）. There was no statistical difference in the expression level of p53 between HepG2 and its progeny（t=0.778, P＞0.05）.　Conclusions　 In the present study,growth delay and declined radiosensitivity are confirmed in the progeny of irradiated hepatocarcinoma cells while these changes are not correlated to the p53 expression.　　【Key words】　HepG2 cell line;Radiosensitivity;p53　　肝癌细胞对放射线有相当的敏感性，但肝癌放射治疗后常残留病灶。放射治疗后复发的肝癌细胞的放射敏感性目前并不清楚，本研究拟应用体外实验方法初步评价肝癌细胞照射后存活后代放射敏感性的变化，旨在为临床放射治疗提供参考资料。　　1　材料与方法　　1.1　细胞培养：人肝癌细胞株HepG2为本院消化研究所保存。HepG2照射后存活后代为HepG2经2Gy照射后存活细胞传代15代的细胞。所有细胞在含10%小牛血清的RPMI 1640（GIBCO）培养基中，37℃，5%CO2的培养箱中培养。　　1.2　细胞群体倍增时间（population doubling time,PDT）：指数生长期的细胞经质量浓度为2.5g/L胰蛋