血清标本溶血对血液生化指标的影响.docVIP

血清标本溶血对血液生化指标的影响45 血清标本溶血对血液生化指标的影响；李大磊1，史文华1，刘志峰1,2；1山东省天然药物工程技术研究中心；2烟台大学药学院山东省烟台市264005；摘要：目的探讨血清标本溶血对血液生化检验结果的影；关键词：溶血；生化检验；；Theinfluenceofhemolysiso；LIDa-lei1，SHIWen-hua1，LI；1．ShandongEngineerin 血清标本溶血对血液生化指标的影响 李大磊1，史文华1，刘志峰1,2 1山东省天然药物工程技术研究中心 2烟台大学药学院 山东省烟台市 264005 摘要：目的 探讨血清标本溶血对血液生化检验结果的影响，为药物安全性评价的长期毒性试验数据的分析提供一定的依据。方法 采用全自动生化分析仪检测10份正常大鼠血液标本在溶血前和溶血后血清葡萄糖(GLU)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、总蛋白(TP)、白蛋白(ALB)、肌酐（Cre）、尿素氮(BUN)、胆固醇(CHO)、碱性磷酸酶(ALP)、总胆红素(TBIL)、钙（Ca++）、镁（Mg++）、钠（Na+）、钾（K+）、氯（Cl-）的值，并进行了比较和统计分析。结果 溶血后AST、TBIL 、ALT、K+的值比溶血前的值高，具有统计学意义；Cre的值比溶血前的值低，统计差异显著；GLU、TP、ALB、BUN、CHO、ALP 、Ca++ 、Mg++ 、Na+ 、Cl-的值溶血前后未见明显统计学意义的改变。结论 溶血对血清AST、TBIL 、ALTK+、Cre有明显的干扰影响，提请在分析测定结果时注意溶血情况。 关键词：溶血；生化检验； The influence of hemolysis on sericem biochemical tests LI Da-lei1，SHI Wen-hua1，LIU Zhi-feng1,2 1．Shandong Engineering Research Center for Natural Drugs, Yantai, Shandong 264003 2．School of Pharmacy, Yantai University, Yantai, Shandong 264005 Abstraet：Aim To observe the influence of hemolysis on the results of biochemical tests. Methods The blood collected from the rat divided into 2 samples, one of which is to process hemolysis serum, the other is to process normal serum. The serum biochemical data were detected respectively, which include glucose(GLU), alanine aminotransferase(ALT), aspartate aminotransferase(AST), total protein(TP), albumin(ALB), creatinine(Cre), Blood uric nitrogen (BUN), cholesterol (CHO), alkaline phosphal ase (ALP), total bilinibin(TBIL ), calcium (Ca++), magnesium (Mg++), natrium(Na+), kalium (K+), chlorin(Cl-). The data were treated with paired-test. Resluts The level of serum AST, TBIL, ALT and K+ increased in hemolysis samples than normal. The level of serum Cre in hemolysis samples is lower than normal. There is no significant difference in serum GLU, TP, ALB, BUN, CHO, Ca++, Mg++, Na+ and Cl- . These results indicate that hemolysis could interfere the results of serum AST, TBIL, ALT, K+ and Cre. The significance of hemolysis on serum biochemical ana