ThreeMacrobrachiumSpecies.PDFVIP

动 物 学 研 究 2009，Dec. 30(6):613-619 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI：10.3724/SP.J.1141.2009.06613 Mitochondrial COI Gene Sequence Variation and Taxonomic Status of Three Macrobrachium Species ZHANG Qing-yi1, CHENG Qi-qun2,*, GUAN Wei-bing1 (1. The Key Laboratory for Oceanic Fisheries Resources Exploitation of Shanghai Education Commission, College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China; 2. The Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China) Abstract: Freshwater prawns (Decapoda: Caridea: Macrobrachium) play an important role in domestic fishery resources. Culturing M. rosenbergii and M. nipponense brings great economic benefits, as the two species were widely farmed in China. M. qilianensis, a native species with natural distribution limited in Gansu province, was classified into genus Macrobrachium based on external morphological characters. In order to understand the molecular genetic differences among the three species of Macrobrachium, i.e., M. rosenbergii, M. nipponense, and M. qilianensis, we analyzed sequences of mitochondrial cytochrome oxidase subunit I (COI) of them. It would provide theoretical basis of exploiting and utilizing Macrobrachium resources rationally. A total of 30 individuals (10 individuals of each species) were collected from Gansu and Zhejiang province. Samples of M. qilianensis were wild, however, that of M. rosenbergii and M. nipponense were cultured. Their mitochondrial COI gene segment sequences were obtained by using the method of PCR amplification and sequencing. After alignment, 649 bp c