Box-Behnken响应面法优化大蒜油提取工艺.pdfVIP

Box-Behnken响应面法优化大蒜油提取工艺Δ 康冰亚 1＊，刘瑞新 1 #，桂新景 2，段晓颖 1，李学林 1，徐立然（3 1.河南中医学院第一附属医院国家中医药管理局中药 制剂三级实验室，郑州 450000；2.河南中医学院药学院，郑州 450046；3.河南中医学院第一附属医院艾滋病 研究中心，郑州 450000） 中图分类号 R284.2 文献标志码 A 文章编号 1001-0408（2017）01-0103-04 DOI 10.6039/j.issn.1001-0408.2017.01.27 摘 要 目的：优化大蒜油的提取工艺。方法：以大蒜油提取率为指标，在单因素试验基础上采用 Box-Behnken 响应面法对水蒸 气蒸馏法提取大蒜中大蒜油的发酵时间、料液比、发酵温度等条件进行优化研究，对优化工艺进行验证试验。结果：最优提取工艺 ∶ 为发酵时间 4.5 h、料液比 1 7、发酵温度 55 ℃。验证试验中大蒜油平均提取率为 0.32％（RSD＝1.43％，n＝3），实测值与预测值之 间的相对误差为 0.06％。结论：采用 Box-Behnken 响应面法优化所得大蒜油提取工艺简便、合理、可行，可为大蒜油工业化大生产 提供参考。 关键词 大蒜油；水蒸气蒸馏法；Box-Behnken响应面法；提取工艺；优化 Optimization of the Extraction Technology of Garlic Oil by Box-Behnken Response Surface Method KANG Bingya1，LIU Ruixin1，GUI Xinjing2，DUAN Xiaoying1，LI Xuelin1，XU Liran（3 1.Third Grade Laboratory， State Administration for TCM，the First Affiliated Hospital of Henan Traditional Chinese Medicine University， Zhengzhou 450000，China；2.College of Pharmacy，Henan University of TCM，Zhengzhou 450046，China；3. AIDS Research Center，the First Affiliated Hospital of Henan University of TCM，Zhengzhou 450000，China） ABSTRACT OBJECTIVE：To optimize the extraction technology of garlic oil. METHODS：Using extraction rate of garlic oil as index，based on single factor test，Box-Behnken response surface method was used to optimize conditions of steam distillation method for the extraction of garlic as fermentation time，solid to liquid ratio，fermentation temperature and the verification test were made for the optimized technology. RESULTS：The optimal extraction technology was as follows as fermentation time of 4.5 ∶ h，solid to liquid ratio of 1 7，fermentation temperature of 55 ℃. The average extraction rate of garlic oil in verification test