沼泽红假单胞菌对亚硒酸盐还原脱毒的研究.PDFVIP

微生物学通报 MAY 20, 2011, 38(5): 660?667 Microbiology China ? 2011 by Institute of Microbiology, CAS tongbao@ 研究报告 沼泽红假单胞菌对亚硒酸盐还原脱毒的研究 李子超 肖娜 李昀锴 王丽娜 虞英杰 徐明芳* (暨南大学生物工程系 广东 广州 510632) 摘 要: 主要研究沼泽红假单胞菌对亚硒酸盐还原脱毒作用及其脱毒机理。通过单因子实验、正 交试验, 对影响亚硒酸盐还原脱毒的因素进行研究, 得到沼泽红假单胞菌还原亚硒酸盐的最佳条 件为: 亚硒酸钠添加量是 25 mg/L, 培养的第 5 天接种, 接种量为 15% (W/W)。在该条件下, 对亚 硒酸钠去除率可达 98.2%。研究发现, 亚硒酸盐还原酶主要存在于细胞质, 分子量约为 182 kD, 由 4 个亚基组成。通过透射电子显微镜观察, 菌体表面出现粒径在 5 nm?200 nm 之间的高电子密度 颗粒, 初步表明亚硒酸盐在沼泽红假单胞菌体内被还原成单质硒。 关键词: 沼泽红假单胞菌, 亚硒酸盐, 还原脱毒, 正交优化, 还原酶 The reduction and detoxification to selenite by Rhodopseudomonas palustris LI Zi-Chao XIAO Na LI Yun-Kai WANG Li-Na YU Ying-Jie XU Ming-Fang* (Department of Biotechnology, Jinan University, Guangzhou, Guangdong 510632, China) Abstract: The reduction and detoxification mechanism of sodium selenite by Rhodopseudomonas pal- ustris were studied in this paper. Effect of factors on the reduction and detoxification of sodium selenite based on a single-factor test and orthogonal experiment were investigated. Results showed that selenite removal rate by the reduction and detoxification of Rhodopseudomonas palustris were achieved at 98.2% under optimal conditions with concentration of sodium selenite at 25 mg/L, 15% of inoculums amount (W/W) and cultivation for 5 days. The researches indicated that selenite reductase with the mo- lecular weight of 182 kD was composed of four subunits polymers and distributed in cytoplasm. Re- ducing selenite into selen