铁钉菜乙酸乙酯提取物抗真菌活性的研究.pdfVIP

第 27卷 第 2期 2011年 3月 福建师范大学学报 (自然科学版) Journal of Fujian Normal University (Natural Science Edition) Vol_27 No．2 M ar．2011 文章编号：1000—5277(2011)02—0109—05 铁钉菜乙酸乙酯提取物抗真菌活性的研究 冯美霞 ，林 勇。，刘艳 如 ，张 迪。，郑 怡 (1．福建师范大学生命科学学院，福建 福州 350108； 2．发育与神经生物学福建省高等学校重点实验室，福建 福州 350108； 3．福建省农业科学院食用菌研究所，福建 福州 350014) 摘要：分别采用纸片法、菌饼法及玻片法测定了铁钉菜乙酸乙酯提取物对 3种真菌的抑制作用．实验结 果表明：乙酸乙酯提取物对 3种真菌具有普遍的抑制作用，对绿色木霉的抑菌圈达到2．60 cm；经薄层层析 分离后，得到 7条层析带，用纸片法测定大部分分离带只对 1种真菌显示抑制活性，其中Rf0．40带和Rfo．50 带活性较强。它们对菌丝生长和抱子萌发也有抑制作用，抑制效果与浓度、培养时间有关．此外，对Rf0．4O 带的GC MS分析表明，其组分中的化合物以酯、烷为主． 关键词：铁钉菜；乙酸乙酯；薄层层析 ；抗真菌 中图分类号：Q936 文献标识码：A Antifungal Activities of the Ethyl Acetate Extracts from Ishige okamura Yendo FENG M ei—xia ”，LIN Yong。，LIU Yan—ru ，ZHANG Di。，ZHENG Yi ’。 (1．College of Life Sciences，Fujian Normal University，Fuzhou 350108，China；2．Fujian Key Laboratory of Developmental and Neuro Biology，Fujian Normal University，Fuzhou 350108，China； 3．Institute of Edible Fungi，Fufian Academy of Agricultural Sciences，Fuzhou 350014，China) Abstract：Ethyl acetate extracts of Ishige oamurai Yendo were tested for the efficacy a— gainst three kinds of fungi by paper disc diffusion method，colony disc method and slide method．The results indicated that ethyl acetate extracts could inhibit all fungi tested and the inhibitory zone of Trichoderma viride reached 2．60 cm． Thin layer chromatography (TLC) was used to separate the extracts of ethyl acetate and each band was examined for their anti— fungal activities by paper disc diffusion method．Of 7 bands，most exhibited inhibitory activity only against one kind of fungus．Besides，of which bands of Rf0．40 and Rf0．50 had stronger activity than others．The two bands still inhibit the mycelial growth and spore germination of the fungi and their activities were related to concentration and culture time．Furthermore，the fraction Rfo．4O was dominated by ester and alkyl through GC-MS analysis． Key words：Ishige oamurai Yendo；ethyl acetate；thin layer chromatographic analysis； antifungi 铁钉菜 (Ishige oamurai