分子生物学第四章技术报告.pptx

;;;;;; 4.1. RNATranscription promotion in prokaryotes;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;; Reconstitution of RNApol. the α+α+ δ+ β’ from Rif R E.coli + β from Rif S E.coli The reconstituted RNA polymerase was Rif S , regardless of the origin of the other subunits the α+α+ δ+ β’ from Rif S E.coli + β from Rif R E.coli The reconstituted RNA polymerase was Rif R , regardless of the origin of the other subunits The β subunit is obviously determinant of RifSor RifR;;;;原核生物转录的起始与延伸过程;;;;;;;;;;;;;;;;;;;;;;;;En. Elem;;;;;;;;;;;;;;;;;;;;;;;;;;;completeTIC;F mRNA Transcriptionstarting;;Promoter clearance;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;? Palindromic sequence;;;;;;;;;;;;;;;;;;Cap;;;;;PABP II (poly(A) BindingProtein) Promotion poly(A) elongation;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;U6/U4;;;;;;1;;;;;;;;;;;;;;;;;;;;;;;;;