应用组织芯片技术研究结直肠癌中Lumican的表达及意义的论文.docVIP

　　应用组织芯片技术研究结直肠癌中Lumican的表达及意义的论文 【摘要】 ［目的］ 探讨lumican的表达与结直肠癌发生、发展的关系。［方法］ 构建100对结直肠癌及相应癌旁正常黏膜上皮组织的组织芯片，应用免疫组化方法检测lumican的表达强度。［结果］ lumican在结直肠癌组织中的表达率（84.0％）显著高于癌旁正常黏膜中的表达率（42.6％）（plt;0.01）。lumican在腺癌中的表达率（87.2％）高于在黏液腺癌及印戒细胞癌中的表达率（68.8％）(plt;0.05)。lumican的表达强度与结直肠癌浸润深度之间存在非常显著的正相关（r=0.07959，plt;0.01）及线性关系(χ2=5.2148，plt;0.05)，即随着浸润程度加深，lumican的表达强度逐渐增加。lumican的表达强度与患者的年龄、性别、发病部位、淋巴结是否转移及分化程度无关（pgt;0.05）。［结论］ lumican的异常表达可能是结直肠癌发生的早期事件，与结直肠癌的组织分型和侵袭能力有关，在结直肠癌的发生发展中发挥重要作用。 【关键词】 肠肿瘤 application of tissue chip assay to expression of lumican for colorectal cancer and its significance song hong-jiang1, tong dan-dan2, jin yin-ji2, et al. (1.third affiliated hospital(cancer hospital) of harbin medical university，harbin 150081，china; 2. department of pathology, the medicine college of harbin medical university, harbin 150081, china) abstract: ［purpose］to explore the relationship of lumican expression ethods］ one hundred couples of tissue chips of colorectal cancer tissues and adjacent normal epithelium mucosa tissues incan easured by immunohistochemical method. ［results］ lumican expression ratio in colorectal cancer tissues (84.0%) al epithelium mucosa tissues (plt;0.01). lumican expression ratio in adenocarcinoma (87.2%) ucinous adenocarcinoma and sig-ring cell carcinoma (68.8%), (plt;0.05). there ican expression and invasive depth in colorectal cancer (r=0.07959,plt;0.01), as ican expression intensity increased as invasive depth increased in colorectal cancer. lumican expression intensity didn’t relate to age, sex, tumor location, lymph node metastasis and cell differentiation (pgt;0.05). ［conclusion］ mal-expression of lumican correlates to histopathologic type and invasive capability in colorectal cancer. it may be the early event of colorectal carcinogenesis. key s; lumican; tissue chip 蛋白聚糖作为细胞外基质的重要组成部分能够调节癌细胞的生长和侵袭，在原发灶的生长或转移灶的形成期间，细胞外基质会通过生物合成和降解形成特征性的重塑[1~3]。.小的富含亮氨酸蛋白聚糖（slrp）不仅可以调节细胞外水平衡、影响组织生物力学，同时在细胞迁移、增殖、肿瘤生长、黏附和移行，以及调节生长因子的活性等方面也发挥着重要作用[4~6]。lumican作为