正常和6羟多巴胺毁损大鼠脚桥核神经元对胆碱能M受体刺激的反应的论文.docVIP

　　正常和6羟多巴胺毁损大鼠脚桥核神经元对胆碱能M受体刺激的反应的论文 【摘要】 目的 在体水平上观察选择性胆碱能m受体激动剂氧化震颤素(oxom)对正常和6羟基多巴胺(6ohda)毁损大鼠脚桥核(ppn)神经元自发电活动的影响。方法 采用玻璃微电极细胞外记录和ppn内注射oxom，观察ppn神经元电活动的变化。结果 与正常组大鼠相比，6ohda毁损组大鼠ppn神经元的平均放电频率增高(plt;0.01)，神经元活动趋于不规则(plt;0.001);ppn内注射oxom对正常组和6ohda毁损组大鼠的ppn神经元放电频率均可产生升高、降低和无变化三种改变。然而，ppn内注射oxom对正常组大鼠ppn神经元的平均放电频率无明显影响，但oxom使6ohda毁损组大鼠ppn神经元的平均放电频率显著降低(plt;0.001)。结论 胆碱能m受体激动剂抑制损毁组大鼠ppn神经元的活动，其作用机制是复杂的，涉及到ppn内突触前和突触后胆碱能受体。 【关键词】 脚桥核;m型胆碱能受体;氧化震颤素;帕金森病;电生理学 　　 abstract: objective to explore the effects of oxotremorine m (oxom), muscarinic acetylcholine receptor agonist, on spontaneous firing rate of neurons of pedunculopontine nucleus (ppn) in normal and 6hydroxydopamine (6ohda)lesioned rats.methods change in the firing activity of ppn neurons in the ppn.results the results shoean firing rate of ppn neurons in the lesioned rats al rats (plt;0.01), and that the firing pattern of the neurons changed toore irregular firing after the lesion (plt;0.001). intrappn injection of oxom induced increase, decrease or no change in the firing rate of ppn neurons in both the normal and 6ohdalesioned rats. hoean firing rate of ppn neurons in normal rats remained unaltered by the stimulation of m receptors, but the mean firing rate of ppn neurons in the lesioned rats decreased significantly by intrappn injection of oxom (plt;0.001).　conclusion m receptor agonist inhibits the activity of the ppn neurons in lesioned rats; the action mechanism of the agonist is plicated, involving not only postsynaptic but also presynaptic m receptors located in different sites acetylcholine receptor; oxotremorine; parkinsons disease; electrophysiology 　　脚桥核(pedunculopontine nucleus, ppn)是一中脑的神经结构，由胆碱能和非胆碱能神经元组成[1]。.解剖学和电生理学研究表明ppn与基底神经节有丰富的交互纤维联系，在运动调控中起重要作用[1]。ppn的主要传入纤维包括来自黑质网状部(substantia nigra pars reticulate, snr)和脚内核(endopeduncular nucleus, ep)的γ氨基丁酸能神经纤维，以及来自底丘脑核(subthalamic nucleus, stn)和额叶皮质的谷氨酸能神经纤维[12]。ppn的上行传出纤维主要投射到黑质致密部(substantia nigra pars pact, snc)、st