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AutoantibodySignatureinHumanDuctal.ppt
Autoantibody Signature in Human Ductal PancreaticAdenocarcinoma;1. Introduction;2. Methods; 3. Materials;4. Experimental Process;Protein lysate;5. Results;;Figure 2.
Validation of PDAC serum reactivity against COF1, TPIS, and K1C10 by WB analysis. The reactivity against COF1 by PDAC sera (N ) 19) was tested on recombinant GST-COF1 by 1-D WB.
(A) Representative result of 1-D WB with anti-cofilin-1 antibody, line 1, and with a PDAC serum, line 2.
(B) Cropped images from CF-PAC-1 2-DE and 2-DE WB with anti-TPIS, anti-K1C10, and anti-COF1 antibody.;;5.2 Expession of identified proteins in tumor and normal pancreatic tissues;6. Discussion; In our study, antibodies to the identified proteins were not detected in sera from HS and non-PDAC tumor patients, suggesting that the antibody response to these proteins is characteristic of PDAC.
As the most frequent antibody response in PDAC
patients is directed to COF1 and TPIS2, our data support the hypothesis that the two proteins play a role in the biology of pancreatic cancer.;Thanks for your attention
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