Ceftriaxonesodiuminjection.PDF

International Journal of Pharma Sciences Vol. 2, No. 4 (2012): 84-90 Research Article Open Access ISSN: 2320-6810 HPLC Method development and Validation for the assay of Ceftriaxone sodium injection Narottam Pal 1*, A. Srinivasa Rao2 and Mohd Abdul Hadi 3 1Assistant Professor, Department of Pharmaceutical Analysis, Bhaskar Pharmacy College (JB Group of Educational Institutions), Yenkapally (V), Moinabad (M), R.R.District, Hyderabad-500075, Andhra Pradesh, India 2Professor and Principal, Bhaskar Pharmacy College (JB Group of Educational Institutions), Yenkapally (V), Moinabad (M), R.R.District, Hyderabad-500075, Andhra Pradesh, India 3Assistant Professor, Department of Pharmaceutics, Bhaskar Pharmacy College (JB Group of Educational Institutions), Yenkapally (V), Moinabad (M), R.R.District, Hyderabad-500075, Andhra Pradesh, India * Corresponding author, email: narotttam_pal868@ Received: 20 October 2012 Revised: 16 November 2012 Online: 16 November 2012 ABSTRACT Ceftriaxone sodium is a third-generation cephalosporin antibiotic. Like other third-generation cephalosporins, it has broad spectrum activity against Gram positive and Gram-negative bacteria. In most cases, it is considered to be equivalent to cefotaximein in terms of safety and efficacy. In the present study an HPLC method was developed and validated for the assay of ceftriaxone sodium injection. The chromatographic system was equipped with Xterra C18 (4.6 x 150mm, 5 ?m, Make:Waters) column and detector set at 242 nm, in conjunction with a mobile phase of Disodium hydrogen phosphate buffer and Acetonitrile in the ratio of 65:35 (pH 4.3,