宫颈病变标本中人乳头瘤病毒感染情况分析的论文.docVIP

　　宫颈病变标本中人乳头瘤病毒感染情况分析的论文 宫颈病变标本中人乳头瘤病毒感染情况分析 【关键词】 乳头状瘤病毒感染;宫颈上皮内瘤样病变;宫颈炎;宫颈肿瘤 【摘要】 目的 检测妇科宫颈标本中人乳头瘤病毒(hpv)感染情况,探讨hpv不同基因型感染的致癌性，为临床早期诊断宫颈癌提供实验依据。方法 首先采用液基薄层细胞学技术(tct)筛查各种宫颈标本，对可疑宫颈病变者(慢性宫颈炎、宫颈上皮内瘤变和宫颈癌)再采用基因杂交捕获技术进行hpvdna检测, 并根据结果进行风险分析。结果 tct共筛查出各种宫颈病变标本460例，包括慢性宫颈炎303例、宫颈上皮内瘤变(cin)122例(其中cinⅰ30例，cinⅱ45例，cin ⅲ 47例)和宫颈癌35例。460例受检者治疗前宫颈hpv感染者211例，感染率为45.87%。慢性宫颈炎、cin和宫颈癌hpvdna阳性率分别为27.06%、77.87%和97.14%，慢性宫颈炎和cin、宫颈癌标本中的hpvdna阳性率比较差异有统计学意义(χ2=79.396，plt;0.01);而cinⅰ、ⅱ、ⅲ组间hpvdna阳性率差异无显著性(χ2=1.21，pgt;0.05)。宫颈病变标本中常见的hpv感染基因型为 hpv16、52、58、68、18、39等。宫颈癌前病变及宫颈癌的hpvdna含量高于慢性宫颈炎，差异有显著性(t=5.581、7.653，plt;0.05);宫颈癌的hpvdna含量也明显高于宫颈癌前病变(t=6.624，plt;0.05); cinⅰ～ⅲ的hpvdna含量呈递增趋势，但差异无显著性(r=0.991，pgt;0.05)。.结论 hpv感染在宫颈病变中起重要作用，对经阴道细胞学筛查异常和高危妇女进一步检测hpvdna,对早期发现cin和宫颈癌具有临床意义。 【关键词】 乳头状瘤病毒感染;宫颈上皮内瘤样病变;宫颈炎;宫颈肿瘤 an analysis of human papilloma virus infection in cervical specimen tang tao, hu xiaofen, zhang ent of gynecology, the affiliated hospital of qingdao university medical college, qingdao 266003, china); [abstract] objective to detect the human papilloma virus (hpv) infection in specimens of cervix in gynecology patients, explore the carcinogenicity of different genotypes of hpv infection, and provide experimental evidence for early diagnosis of cervical cancer. methods firstly, the thincytologic test (tct) ens of cervix, for doubtful specimens, such as chronic cervicitis (cc), cervical intraepthelial neoplasia (cin) and cervical cancer, a secondgeneration hybrid capture 2 (hc ⅱ) for hpvdna detection and highrisk analysis ples ong cc, cin and cancer ong cinⅰ, cinⅱ and cinⅲ only seen genotypes of hpv infection in the cervix ong themt (r=0.991,pgt;0.05). conclusion hpv infection plays an important effect on the pathological changes of cervix, for those al colpocytology findings and highrisk en, a further detection of hpvdna is of great significance in early discovery of cin and cervical cancer. 　　[key avirus infection; cervical intraepithelial neoplasia; cervicitis;