3GalT7 transfected HL60 cells and their biological behavior.docVIP

 PAGE \* MERGEFORMAT 13 3GalT7 transfected HL60 cells and their biological behavior OF: Script Li, Yue love ring, Tang Chun-hua, Jia Wei, WU Shi-liang] [Abstract] Objective: To investigate the human 1, 3 galactosyltransferase enzyme 7 (3GalT7 gene on HL60 cell cycle, invasion and so on. Methods: Liposome mediated by the laboratory for good 3GalT7 eukaryotic expression vector of justice pEGFP C1 T7 Sense and antisense expression vector pEGFP C1 T7 AntiSense transfected into HL60 cells, the cells analyzed by flow cytometry cell cycle changes, Transwell invasion assay 3GalT7 on HL60 cell invasion and metastasis of . Results: The expression of increased 3GalT7, G2 + S phase cells increased, and enhanced cell invasion, while the antisense expression vector pEGFP C1 T7 AntiSense result is the opposite. Conclusion: Overexpression of the HL 60 cell line 3GalT7 Enhanced in vitro proliferation and invasion. [Keywords:] 1, 3 galactosyltransferase enzyme 7, sense expression vector, antisense expression vector, cell cycle, invasion [Abstract] Objective: To explore the effects of 3GalT7 gene on cell cycle, invasive potential of HL 60.Methods: Transfect the recombinant plasmids pEGFP C1 T7 Sense and pEGFP C1 T7 AntiSense into HL 60 with liposomes.Cell cycle and invasive potential of the transfected cells with flow cytometry and transwell plate, respectively. Results: As the up regulation expression of 3GalT7, the cell growth rate increased. At 48 h after transfection, significantly increased invasiveness was noted in HL60 transfected with pEGFP C1 T7 Sense. Conclusion: Over expressing 3GalT7 in HL 60 enhanced proliferative and invasive potential. [Keywords:] beta 1, 3 galactosyltransferase 7, plus sense recombinant plasmid, antisense recombinant plasmid, cell cycle, invasive potential 1, 3 galactosyltransferase gene family is the glycosyltransferase superfamily one of the most representative family of enzymes, 1, 3 galactosyltransferase enzyme 7 (beta1, 3 ga