4 1BBL gene transfer of mouse liver cancer cell vaccine to stimulate the spleen cells to produce cytokines of.docVIP

 PAGE \* MERGEFORMAT 12 4 1BBL gene transfer of mouse liver cancer cell vaccine to stimulate the spleen cells to produce cytokines of Author: Liu Chengli, Zang Xiaoxia, Dou Ke-Feng, Zhu Gang-fu, Zhang Hongyi, Zhang Hongyi 【Abstract】 Objective To study the turn 4  1BBL gene vaccine in mice hepatoma cells in vitro stimulated syngeneic mouse spleen cells to produce cytokines (IL  2, TNF  α and GM  CSF) capabilities. Ways to mitomycin C (MMC) treatment of high expression of m4  1BBL gene transfer in mice Hepa1  6 liver cancer cell vaccine made of tumor cells (TCV), in vitro and in mice with spleen lymphocytes co-cultured, observe the effect of spleen cells to produce cytokines (IL  2, TNF  α and GM  CSF) effects. Results TCV  4  1BBL stimulated spleen cells in vitro secretion of cytokines IL  2, TNF  α and GM  CSF levels increased significantly. Conclusion 4  1BBL gene transfer in mice liver cancer cell vaccine can stimulate the spleen cells to produce cytokines IL  2, TNF  α and GM  CSF. Keywords: Mouse 4  1BBL; co-stimulatory molecules; tumor vaccines; hepatocellular carcinoma; cell factor Abstract: Objective To study the cytokines production of spleen cells induced in vitro by murine 4  1BBL gene transfected Hepa1  6.Methods Murine hepatocellular carcinoma cell line Hepa1  6  m4  1BBL which could highly express murine 4  1BBL was cultivated previously. The tumor cell vaccine (TCV  m4  1BBL) was obtained by treating Hepa1  6  m4  1BBL with mitomycin (MMC). Cocultivation TCV with syngeneic murine spleen cells and the supernatants were harvested for detecting the cytokines (IL  2, TNF  α and GM  CSF). Results Comparing with TCV  Hepa1  6, high levels of IL  2, TNF  α and GM  CSF were released by the spleen cells after stimulated by TCV  m4  1BBL.Conclusion Stimulation signals delivered by 4  BBL remarkably increased the production of IL  2, TNF  α and GM  CSF. Keywords:: murine 4  1