62 cases of laryngeal cancer patients in high-risk HPV (16-18 type) gene analysis.docVIP

 PAGE \* MERGEFORMAT 11 62 cases of laryngeal cancer patients in high-risk HPV (16/18 type) gene analysis Of: Peng Zheng Jian, Gengjian Xiang, Zhou, Fu Xuedong [Keywords:] laryngeal squamous cell carcinoma; HPV; high risk; in situ hybridization High risk human papilloma virus (human papilloma virrus, HPV) is the main cause human cervical cancer is known to cause human cancer is currently the only incentive to clear the virus. Squamous cell tropism according to the characteristics of HPV, the authors used in situ Detection of HPV gene hybridization technique to detect squamous cell carcinoma of the larynx, of high-risk type (type 16/18) HPV expression and the relationship between laryngeal squamous cell carcinoma, to explore its clinical significance. 1 Materials and methods 1.1 The group of 62 specimens from selected cases of Nanjing University of Traditional Chinese Medicine Department of Pathology, Third Affiliated Hospital, Anhui Province and the First People’s Hospital, Department of Pathology, First People’s Hospital Chuzhou Pathology in April 2004 on October -2008 standard surgical resection of the larynx between the 62 patients were male patients. aged 43 to 82 years, mean 65 years. All the cases no preoperative radiotherapy and chemotherapy, patients with pathologically confirmed cases of laryngeal .62, well differentiated carcinoma in 24 cases, undifferentiated carcinoma in 17 cases, 21 cases of poorly differentiated carcinoma; 27 cases of supraglottic, glottic 35 cases; 21 cases of clinical stage , in 23 cases, of 12 cases, 6 cases; lymph node metastasis in 21 cases, 41 cases without lymph node metastasis. 1.2 Detection Methods 62 patients with laryngeal cancer and 15 cases of laryngeal squamous epithelial tissues of normal (as control specimens), specimens were fixed 10% formalin, embedded in paraffin, 4m sections, routine HE staining, the line in situ Hybrid HPV (16/18 type) DNA testing. digoxigenin mind HPV (16/18 type) probe,