8 - nitro-chrysin on the HL-60 cell apoptosis and PTEN-Akt Pathway.docVIP

 PAGE \* MERGEFORMAT 11 8 - nitro-chrysin on the HL-60 cell apoptosis and PTEN-Akt Pathway [Abstract] Objective To observe the 8 - nitro-chrysin (NOChR) inhibit acute myeloid leukemia cell line (HL-60) cell proliferation and induce apoptosis and to explore its mechanism whether the PTEN-Akt signal transduction pathway related. The cells were cultured HL-60 cells. Proliferative activity of MTT colorimetric assay; flow cytometry (FCM) Quantitative analysis of the extent of apoptosis; Western Blot analysis of HL-60 cells, PTEN, P-Akt protein expression. Results NOChR significantly inhibited the proliferation of HL-60 cells in a dose-dependent manner, its IC50 value of 1.34 μ mol / L; NOChR can induce apoptosis of HL-60 cells, and the dose-and time-dependent. NOChR a concentration-and time-dependent manner HL-60 cells increases PTEN protein expression and inhibit P-Akt protein expression and its role could be PPARγ specific antagonist GW9662 (10 μ mol / L) pre-incubated with antagonist. Conclusion NOChR induced apoptosis in HL-60 cells and its activation of PPARγ , increase PTEN protein expression, inhibit inhibit Akt phosphorylation. [Keywords:] human acute myeloid leukemia chrysin apoptosis Chrysin (ChR) is a kind of broad biological activity of flavonoids, in the higher levels of propolis, has anti-oxidation, anti-virus, anti-hypertension, anti-diabetic and other pharmacological effects. Previous experimental studies have shown that, ChR inhibit human thyroid cancer cell growth; through the estrogen receptor antagonist, inhibit human breast cancer cell DNA synthesis; through the regulation of tumor necrosis factor-α (TNF-α ) expression induced apoptosis in human hepatoma cells. Our study found that 8 - nitro-chrysin (NOChR) Inhibition of human gastric cancer (SGC-7901) cells, human colon cancer (HT-29) cells, the role of chrysin stronger than [1]. Song et al [2] reported that chrysin can inhibit the protein kinase CK2, increase the product of PTEN tumor sup