About Disporopsis angiogenesis inhibition in the active ingredients with the screening.docVIP

 PAGE \* MERGEFORMAT 11 About Disporopsis angiogenesis inhibition in the active ingredients with the screening OF: He waves washed Mengmai Xia Yang Zhifeng [Abstract]: Objective Disporopsis in human umbilical vein endothelial cells (ECV304) inhibited the growth of the active ingredients. Methods seven solvent extraction method for different medicinal parts of bamboo root, to anti-vascular endothelial growth factor (VEGF) antibody as control, employing human umbilical vein endothelial cell membrane chromatography model of screening, and screening results were analyzed with the MTT test to determine which of the active ingredients initially. Results Disporopsis separation of components (ZGQ-C) concentration in were 0.625,1.25,2.50,5.00,10.0 μg / mL, the proliferation of ECV304 inhibition rates were 40.51%, 51.11%, 63.54%, 74.32%, 81.26%, half of the inhibition rate of 1.00 ~ 1.25 μg / mL range , the same cells observed under the microscope the form of significant change. Conclusion ZGQ-C is inhibited ECV304 certain active ingredients, human umbilical vein endothelial cell membrane chromatography model of the screening results between the experimental results with the MTT better relevance. [Keywords:] Disporopsis inhibiting angiogenesis ECV304 cell membrane chromatography Abstract: Objective To discover the effective component of Rhizoma Tupistrae chinensis with inhibiting angiogenesis. Methods Several parts of Rhizoma Tupistrae chinensis were obtained by using different extraction solvents. They were screened by the model of ECV304 cell membrane chromatography with anti-VEGF antibody as a control drug. The inhibition of effective component (ZGQ-C) on ECV304 proliferation in vitro was examined by MTT assay. Results The inhibition rate (40.51%, 51.11%, 63.54%, 74.32%, 81.26%) was correlated with the ZGQ-C concentration. The ECV304 cell had significantly changed in morphology. Conclusion The ZGQ-C is an effective component for inhibiting angiogenesis. Th