About Eiemene of human cervical carcinoma Hela cell transcription factors and target genes of ELK1.docVIP

 PAGE \* MERGEFORMAT 9 About Eiemene of human cervical carcinoma Hela cell transcription factors and target genes of ELK1 Of: Chen Xiaojun Gu Ligang Li Peiwen Shi Taiping Mada Long [Abstract: Objective To observe the effect of elemene emulsion on human cervical carcinoma Hela cell transcription factors and target genes of ELK1 to learn more about Eiemene anticancer mechanism. Methods MTT assay the inhibitory effect of drugs on cells dual luciferase reporter system for transcription factor ELK1 detection of drug effects, Western Blot detection of phosphorylated ELK1 and its target gene c-fos expression. Results Eiemene can inhibit the growth of human cervical cancer Hela cells, the half of the growth inhibitor content of 80.6 μg / mL, Eiemene treatment decreased luciferase activity, phosphorylation of ELK1 and its target gene c-fos expression were decreased. Conclusion Eiemene possibly through reduced phosphorylation of transcription factor ELK1 level, inhibition of c-fos expression, thereby inhibiting the growth of human cervical cancer Hela cells. [Keywords: Eiemene Hela cells ELK1 Abstract: Objective To study the effect of elemene on transcription factor ELK1 and its target gene in human cervix cancer Hela cell. Methods The cell proliferation was assessed by MTT assay. The luciferase activity of transcription factor ELK1 was determined by the Dual-Luciferase Reporter Assay System. The protein expression of phosphorated ELK1 and its target gene c-fos were determined by Western Blot. Results Elemene can remarkably inhibited the growth of Hela cell and its IC50 was 80.6 μg / mL. The luciferase activity of transcription factor ELK1 in Hela cells treated with elemene was inhibited. The protein expression of phosphorated ELK1 and its target gene c-fos in Hela cells treated with elemene were down-regulated. Conclusion Elemene can inhibit human cervix cancer Hela cell proliferation, which may be related with suppression of c- fos gene through inhibiting e