About Qidantongmai tablets combined with bone marrow mesenchymal stem cell transplantation in acute myocardial ischemia on myocardial apoptosis and cardiac function.docVIP

 PAGE \* MERGEFORMAT 13 About Qidantongmai tablets combined with bone marrow mesenchymal stem cell transplantation in acute myocardial ischemia on myocardial apoptosis and cardiac function Of: Wang, EMERGENCY, EMERGENCY IN TRADITIONAL Hai gravel, Wang Wenyong, Mafu Cheng, Jing [Abstract] Objective To observe Qidantongmai tablets combined with bone marrow mesenchymal stem cells (MSCs) transplantation on rats with acute myocardial apoptosis, heart function. Methods were isolated by density gradient centrifugation of rat MSCs, expanded to passage 3, the application of bromodeoxyuridine (BrdU) labeled cells .16 healthy SD rats were randomly divided into combined transplantation Qidantongmai Tablet group simple transplantation group were fed with dry powder extracts Qidantongmai tablets solution (2 g / kg) and volume of saline, day 1, continuous 14 d. Left anterior descending coronary artery ligation, preparation of acute myocardial infarction model, allogeneic MSCs labeled with BrdU microinjection method of transplantation into the ischemic myocardium. Transplanted 3 h after the application of HP5500 ultrasonic diagnostic apparatus, the probe frequency of 12 MHz, testing large rat heart function of 4 weeks and used immunofluorescence ischemia microvessel density (MVD), using deoxynucleotidyl transferase (TdT enzyme)-mediated dUTP nick end labeling (TUNNEL) withered myocardial cells apoptosis index (AI). Results Qidantongmai tablets transplantation group and the simple transplantation group had improved heart function, and transplantation Qidantongmai tablets group improved more significantly. The AI #8203;#8203;was significantly reduced compared with transplantation alone, the ischemic region significantly increased MVD. Conclusion Qidantongmai tablets plus MSCs transplantation can improve myocardial ischemia in rats with acute heart function, reduced cardiac myocyte apoptosis, increased ischemic area MVD, compared with MSCs transplantation alone more prono