About quercetin on proliferation of human breast cancer cells.docVIP

 PAGE \* MERGEFORMAT 12 About quercetin on proliferation of human breast cancer cells Study: Pi-Shen Zhao Wen Xuhui Yu Lixia Zhao WANG Ji-feng Niu [Abstract]: Objective quercetin (Quercetin, Que) on the proliferation of human breast cancer cell lines. Methods MTT method using quercetin for estrogen-dependent breast cancer cells MCF-7 and non-estrogen-dependent breast cancer cells MDA-MB231 cell proliferation, and estrogen receptor antagonist ICI 182,780 as a tool to evaluate drug quercetin to play the role of estrogen and estrogen-like receptor (ER) relationship, using flow cytometry on the proliferation of MCF-7 cells were analyzed. The results compared with the control group, Que (10 ~ 50 μmol / L) could significantly promote the proliferation of MCF-7 cells, and inhibition of ER-negative MDA-MB231 cells and MCF -7 cell cycle from G1 to S phase of advancing, promoting DNA synthesis, increased cell proliferation index, and Que promote proliferation of MCF-7 cells were antagonized by ER antagonists. Conclusion Que has estrogenic activity, this effect is through ER mediated. [Keywords:] quercetin phytoestrogens estrogen receptor cells MCF-7 MDA-MB231 Abstract: Objective To investigate the effects of quercetin on the proliferation of mammary cancer cell in vitro. Methods Effects of quercetin on the cell proliferation was tested in ER-positive MCF-7 cell and ER-negative MDA-MB231 cell by MTT measurement. And to evaluate the estrogen-like effect of quercetin and its relation with the estrogen-receptor by pure estrogen receptor antagonist ICI 182, 780 as a tool. Cell cycle was examined by flow cytometry. Result Quercetin (10 ~ 50 μmol / L) stimulated proliferation of ER-positive MCF-7 cell compared with solvent control, whereas ER-negative MDA-MB231 cell proliferation effect was inhibited, and the cell cycle was impulsed from G1 to S, DNA synthesizing was inhanced, PI was also increased. The above function on boosting MCF-7 cell proliferation can be inhi