About RP HPLC determination of Salvia comfortable capsule contents Sal B.docVIP

 PAGE \* MERGEFORMAT 6 About RP HPLC determination of Salvia comfortable capsule contents Sal B [Abstract] RP HPLC method for the determination comfortable Capsules Salvia Sal B levels. Stationary phase Kromasil C18 reversed phase column; mobile phase of methanol formic acid water (10:30:1:59); detection wavelength 286nm; flow rate: 1.0 ml / min. The results showed that Sal B on the average recovery was 100.05%, RSD = 0.38% (n = 9), r = 0.9998. [Keywords:] RP-HPLC; Danshen Shuxin Capsule; Sal B Salvia Extract Salvia comfortable capsules made of capsules, with the blood circulation, sedative effect, patients are used to treat angina caused by coronary heart disease, chest tightness and palpitations. The admission criteria set out in the Ministry of Health Pharmaceutical prescription formulations in the first seven Chinese. The standard is no record determination. In this study, using high performance liquid chromatography Sal B were determined, the results show that the law is specific, reproducible, and can be used as content Determination of the quality control indicators. 1 Materials and Manufacturing 1.1 Instrument and reagents LC 10ATVP high performance liquid chromatograph (Shimadzu); SPD 10AVP UV detector; N2000-based chromatography data workstation; 92SM 202ADR electronic balance; V 530 UV spectrophotometer; Sal B standard (China Pharmaceutical Biological Products, lot number 111562200807); Danshen comfortable capsules (commercially available products, Henan, Fu Jen Catholic Church Pharmaceutical Co., Ltd.). Acetonitrile, methanol, formic acid was HPLC grade water as purified water. 1.2 Chromatographic conditions Column: Kromasi C18 reversed phase column (4.6 mm × 250mm, 5μm); column temperature: room temperature; mobile phase: acetonitrile methanol formic acid water (10:30:1:59); detection wavelength 286nm; injection volume 10μl; theory plate number calculated by Sal B peak is not less than 4000; separation is greater than 1.5.