Acetochlor carcinogenic evaluation of pathology tests.docVIP

 PAGE \* MERGEFORMAT 10 Acetochlor carcinogenic evaluation of pathology tests Keywords:: acetochlor toxicity Acetochlor (Acetochlor) is a selective herbicide bud dry land, mainly for corn, soybeans, cotton, sugar cane and a variety of vegetable, mu 50 ~ 80 g once a pesticide to control weeds throughout the growing season no harm, is to promote the value of a new herbicide. Acute toxicity oral, percutaneous LD50 were 2 148 mg / kg, 4 166 mg / kg, SD rats were fed 90 d subchronic sigh no role in the biggest test dose of 12 mg * kg-1 .. d-1, Ames, micronucleus and testicular chromosome test results were negative. We further made a two-year carcinogenicity tests, are reported as follows. 1 Materials and methods 1.1 Acetochlor 95% of samples tested by the chemical plant in Kunshan, Jiangsu Province, Nantong chemical plants, pesticide plants in Dalian, Liaoning Province, Hebei Province, to provide Xuanhua pesticide plant in batches. 1.2 Animal health, clean-level 5-week-old SD rats were provided by Shanghai BK, animal quarantine, domestication started 1 week delivery. Set up a control group, 200,700,2 000 mg / kg * feed three dosage groups, daily food intake by rats dose equivalent conversion 12,42,120 mg * kg-1 .. d-1. Application of computer-generated random groups, each group of 48 male and female, a total of 384. 1.3 Animal feeding trials barrier system in the whole enclosed within. Within its system average temperature (22 ± 4) ℃ , humidity (55 ± 10)%, about 200 lx illumination, the air through the early, middle and high 3 filtering (filtration rate of 99.97%), ventilation from 12 to 16 times / h , the system to maintain slightly positive pressure, noise 60 dB below the ammonia concentration of 20 mg / kg the following. Automatic drinking device for animals to drink. Animals in a cage hanging aluminum single-cage rearing. Feed by 60Co irradiation of sterile powder. Free feeding animals during the test water. 1.4 Clinical observation of weight 0 ~ 13