Acid molecularly imprinted membrane electrochemical biosensor.docVIP

 PAGE \* MERGEFORMAT 13 Acid molecularly imprinted membrane electrochemical biosensor Of: Li Huai-Fen Hsieh Cheng-Gen Xie Chunyan Zhou Hankun [Abstract] salicylic acid as the template molecule, using cyclic voltammetry, the formation of polypyrrole electropolymerization to a fixed potential of molecular imprinting removed by peroxide, salicylic acid was prepared by molecular imprinting membrane electrode. The imprinted electrode can promote the water Yang acid electro-oxidation process, effectively avoid structural analogs (such as the determination of benzoic acid to its interference. Cyclic voltammetry for electrochemical detection, when the enrichment time was 10 min, phosphate buffer solution of pH = 6.86 when In the 1.0 × 10-6 ~ 2.0 × 10-3 mol / L concentration range, the oxidation peak current and the concentration of salicylic acid showed a good linear relationship, the detection limit was 0.8 μmol / L, with the increase of molecular imprinted membrane electrode Standard samples were analyzed, recoveries were 94.6% ~ 103.4%. [Keywords:] salicylic acid, molecular imprinting, electro pyrrole, modified electrode, the sensor Abstract Molecular imprinted film modified glassy carbon electrode was prepared by electrochemical polymerization of pyrrole with the cyclic voltammetry in the presence of template molecular, salicylic acid. The template molecules were removed from the modified electrode surface by over oxidized at 1.3V in the solution of 0.2 mol / L Na2HPO4 for 10 min. The modified electrodes can effectively promote electrochemical oxidation process of salicylic acid in the surface of electrode and avoid interference of structural similar substance such as benzoic acid. Cyclic voltammetry was employed in the electrochemical measurements. The experimental results show that the optimum acidity of background solution is pH 6.86 and the optimum incubation is 10 min. The linear response curve was obtained from 1.0 × 10-6 mol / L to 2.0 × 10-3 mol