Acid on human normal esophageal mucosal epithelial cell proliferation and ERK expression in.docVIP

 PAGE \* MERGEFORMAT 7 Acid on human normal esophageal mucosal epithelial cell proliferation and ERK expression in Author: Jiang Zhiru, Gong Jun, Zhang Jenny, Wenbin Zeng [Keywords:] acid; esophagus / cytology; cell proliferation; extracellular signal-regulated MAP kinase Influence of bile acid on proliferation and ERK expression in human normal esophageal epithelial cells [Abstract] AIM: To observe the influence of bile acid on cell proliferation and the expression of extracellular signalregulated protein kinase (ERK) in human normal esophageal epithelial cells in vitro. METHODS: Human normal esophageal epithelial cells cultured in vitro were exposed to media containing 6 different kinds of bile acids (250 μ mol / L) respectively for 360 min, in comparison with the control group (in normal media without bile acid). Cell proliferation was assessed using MTT and flow cytometry. The expression of phosphorylated ERK1 / 2 protein was determined by the immunoblotting technique. RESULTS: Bile acidexposed (312 min) esophageal epithelial cells exhibited a significant increase in proliferation, especially in S phase of the cell cycle, and the level of phosphorylated ERK1 / 2 protein. While the bile acidexposed period exceeded 20 min, esophageal epithelial cells exhibited a degression in proliferation and proportion on S phase of the cell cycle. CONCLUSION: The brief exposure in bile acid increases the proliferation in human normal esophageal epithelial cells by activating the ERK pathway. [Keywords] cholic acid; esophagus / cytology; cell proliferation; extracellular signalregulated MAP kinases [Abstract] Objective: To observe the acid on human normal esophageal mucosal epithelial cell proliferation and ERK expression. Methods: The cultured human normal esophageal mucosal epithelial cells, respectively, containing 6 different bile acid (250 μ mol / L) in cultured liquid culture 3 ~ 60 min, and let the control (culture medium without bile acid) were compared. using