Acinetobacter baumannii aminoglycoside-modifying enzyme and type I integrase gene research.docVIP

 PAGE \* MERGEFORMAT 11 Acinetobacter baumannii aminoglycoside-modifying enzyme and type I integrase gene research Author: Chen Lin Tsai Ting Zhang Xu Xiaomin [Abstract] Objective To understand the hospital Acinetobacter baumannii (Acinetobacter baumannii, AB) type I aminoglycoside-modifying enzymes and the presence of integrase gene. Methods According to the United States CLSI / NCCLS 2004 edition of the requirements of antimicrobial susceptibility testing, application of PCR technology to AB bacteria to aminoglycoside-modifying enzymes and type I integrase gene detection. The results of AB pairs of 13 species of bacteria resistance to antimicrobial medicines seriously. 27 AB bacteria in the aminoglycoside-modifying enzyme gene aac (3)  I-positive 23 (85.2%), aac (6 ‘)  I-positive 18 (66.7%), ant (3 amp;quot;)  I-positive 22 (81.5%). aminoglycoside-modifying enzyme gene overall detection rate of 85.2%. I type integrase gene (intI1) positive 23 (85.2%). Conclusion AB in our hospital not only has the multi-resistant bacteria, and aminoglycosides modifying enzyme genes, I type integrase gene carried a high rate. [Keywords:] Acinetobacter baumannii; multi-drug resistance; aminoglycoside-modifying enzymes; I type integrase gene ABSTRACT Objective To study the aminoglycosides modifying enzyme genes and intI gene in Acinetobacter baumannii in Ningbo No.2 Hospital. Methods The susceptibility of 13 antibacterials were analyzed according to CLSI (2004) Standards. The aminoglycosides modifying enzyme genes and intI1 gene were detected by PCR . Results The multi  drug  resistance of A.baumannii was a serious problem. In 27 strains of A.baumannii, the positive strains of aac (3)  I were 23 strains (85.2%); aac (6 ‘)  I were 18 (66.7%); ant (3 amp;quot;)  I were 22 (81.5%) and others were negative. The total detection rate of aminoglycosides modifying enzyme gene was 85.2%. The positive strain of intI1 gene was 23 strains (85.2% ). Conclusions There were