Activated ACM on rotenone-induced inhibition of cellular oxidative damage.docVIP

 PAGE \* MERGEFORMAT 10 Activated ACM on rotenone-induced inhibition of cellular oxidative damage On: The Story of Qiu FORCES Chensheng Peng Liu Hui Yin Fangqiu Liu Jie He high-Sen Abstract Objective To observe the activation of astrocyte conditioned medium (ACM) in PC12 cells induced by rotenone in the role of oxidative injury. Methods activation ACM, exposure to rotenone in PC12 cells was observed on neurons exposed to the malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH Px) activity and detection of cellular ROS (ROS) level and mitochondrial respiratory chain complex activity changes. The results can be significantly reduced ACM PC12 cells exposed to MDA and ROS levels; treatment compared with the complete medium, ACM can effectively increase the exposure of neurons SOD and GSH Px synthesis and release, protect and enhance the activities of antioxidant enzymes. Conclusion ACM can effectively inhibit rotenone-induced oxidative damage in PC12 cells. Keywords: astrocyte conditioned medium; rotenone; oxidative damage Study found that chronic exposure to pesticide rotenone causes Parkinson’s disease is (PD) and other neurodegenerative diseases, one of the important environmental triggers 1. Rotenone is a mitochondrial inhibition pesticides, caused by mitochondrial dysfunction and oxidative stress and dopaminergic neuron damage is closely related to 2, and mitochondrial dysfunction mainly due to complex activity was inhibited. Astrocytes produce neurotrophic factors in vivo primary cells, activated astrocytes conditioned medium (ACM) containing nerve growth factor and glial derived neurotrophic factor and other neurotrophic composition 3 . In this study, ciliary neurotrophic factor (CNTF) activation of astrocytes, the normal collection of ACM treatment and exposed to different concentrations of rotenone in PC12 cells were induced by rotenone ACM on neuronal oxidative injury, in order to clarify the star glial