Acupuncture treatment of gastric ulcer effect of proteomics research platform technology to optimize and Strategy.docVIP

 PAGE \* MERGEFORMAT 17 Acupuncture treatment of gastric ulcer effect of proteomics research platform technology to optimize and Strategy Author: Yang Bo, Gao Yang, Zhang Yan, YAN Xing Branch [Abstract] Objective To carry out acupuncture gastric ulcer proteome research, build and optimize their proteomic analysis of the required two-dimensional gel electrophoresis technology to improve their resolution and repeatability. Methods of acetic acid ulcer rats after acupuncture whichever stomach ulcers Service Organization, the organization to improve the protein extraction method to optimize two-dimensional gel electrophoresis of the key factors and links, such as sample preparation and pre-treatment, first to the IPG isoelectric focusing and electrophoresis of the sample volume parameters, the second vertical plate to the concentration of SDS gels, staining methods. PDQuest7.1 software analysis obtained using two-dimensional gel images. Results are solid-phase pH gradient-IPG strips (pH3 ~ 10) carried out first to isoelectric focusing, to homogeneous SDS gel (12.5%) of the vertical gel electrophoresis as the second direction, succeeded in getting the two groups of rats with gastric tissue distribution of spots uniform, a higher number of two-dimensional gel electrophoresis maps. Conclusion of two-dimensional gel electrophoresis of the establishment and optimization study of gastric ulcer was suitable for high-resolution, high repeatability of the two-dimensional gel electrophoresis maps. [Keywords:] two-dimensional gel electrophoresis SDS gel electrophoresis proteomics Proteomics is the application of technical means to study the proteome of an important discipline, using proteomic analysis of applications and technologies can identify and identification of a cell or tissue expressed all the proteins and their expression patterns, from the overall protein level on in a more in-depth, closer to the essence of life, level of activity up to explore a number of imp