Adenovirus-mediated human wild-type p53 GMCSF and B71 gene expression in pancreatic cancer cells.docVIP

 PAGE \* MERGEFORMAT 15 Adenovirus-mediated human wild-type p53 GMCSF and B71 gene expression in pancreatic cancer cells Of: Tan Yulin, strict country, Xi Jianbo, French town, Tang Jianjun, Wang Geng [Abstract] Objective: The recombinant adenovirus as a carrier, the use of human wild-type p53 gene, human GM CSF gene and the gene for the purpose of B7 1 gene of the three genes in pancreatic cancer cells BxPC 3 expression. METHODS: Immunohistochemical histochemical detection of exogenous p53 protein expression in BxPC 3 cells. ELISA method for determination of GM CSF expression, flow cytometry (flow cytometry, FCM detection of BxPC 3 cells transfected with B7 1 expression results. Results: The recombinant adenovirus mediated p53 gene, GM CSF gene, B7 1 gene in BxPC 3 cells was highly expressed. Conclusion: Adenovirus-mediated foreign genes in BxPC 3 cells have high expression, in order to further investigate the in vivo treatment Experimental study of pancreatic cancer provides a theoretical basis. [Keywords:] pancreatic cancer, gene therapy, adenovirus, tumor suppressor gene p53, granulocyte-macrophage colony-stimulating factor, immune co-stimulatory molecules B7 1 [Abstract] Objective: To study the expression of human wild type p53, GM CSF and B7 1 genes mediatedly adenovirus vetor on human pancreatic carcinoma cell line BxPC 3.Methods: The expression of p53 protein was examined by immunohistochemical analysis. The expression of GM CSF was detected by sandwich ELISA analyses with culture supernatant. The expression of B7 1 was detected with FCM. Results: BxPC 3 cells were transfected with DMEM, Ad GFP, AD WTp53 GMCSF B7 1.The cells transfected with the Ad WTp53 GMCSF B7 1 resulted in positive expressing the wild type p53 protein, GM CSF and B7 1, but not in DMEM and Ad GFP transfected cells. Conclusion: The expression on human pancreatic carcinoma with adenovirus mediated human wild type p53, GM CSF and B7 1 genes was more effective.These results pr