Angiostatin in diabetic nephropathy and MMP2 and the expression of MMP9.docVIP

 PAGE \* MERGEFORMAT 10 Angiostatin in diabetic nephropathy and MMP2 and the expression of MMP9 [Abstract] Objective To investigate the adeno-associated virus-mediated angiostatin gene (rAAV AS diabetic nephropathy (DN rats renal cortex of matrix metalloproteinase 2 (MMP 2 and 9 (MMP 9 expression. Methods Streptozotocin (STZ intraperitoneal injection to establish animal models of diabetes. SD rats were randomly divided into control group, diabetes group, rAAV vector group and rAAV AS treatment group. the first 10 weeks of test rats with microalbuminuria (MAU), and renal hypertrophy index , renal function, blood glucose, glycosylated hemoglobin; immunohistochemistry renal cortex MMP 2, MMP 9, the pathological changes observed under light microscope. Results renal cortex MMP 2, MMP 9 was higher than the diabetic group, but were lower than control group, there is a corresponding immunohistochemistry. Conclusion rAAV AS on the renal protective effect of DN, and its possible mechanism in the renal cortex with increased MMP 2, MMP 9 expression, thus reducing the renal extracellular matrix (ECM ) of the aggregation. [Keywords:] adeno-associated virus-mediated angiostatin gene, diabetes, kidney disease, matrix metalloproteinase 2, matrix metalloproteinase 9 Diabetic nephropathy (DN) is essentially a vascular endothelial disorder syndrome, is the most common chronic diabetic microvascular complications. Glomerular extracellular matrix accumulation and then sclerosis is characteristic of the pathological changes of DN (1). Kidney organizations to promote ECM degradation is the main physiological regulator of matrix metalloproteinases (MMPs, ie MMP 2, MMP 9, which can degrade all known extracellular matrix (2,3). angiostatin (AS could specifically inhibit tumor angiogenesis endothelial cell proliferation factor (4), increased MMP expression, decreased proteinuria DN (5), play a role in renal protection. adeno-associated virus-mediated angiostatin gene (rAAV AS be