Antibody against aflatoxin M1 and Detection Methods.docVIP

 PAGE \* MERGEFORMAT 4 Antibody against aflatoxin M1 and Detection Methods [Keywords:] aflatoxin M1; monoclonal antibody; enzyme-linked immunosorbent assay Abstract: Objective To prepare for aflatoxin M1 and the establishment of monoclonal antibodies against aflatoxin M1 in indirect competitive enzyme-linked immunosorbent assay detection method. Methods B-cell hybridoma technique to establish that secrete monoclonal antibodies against aflatoxin M1 hybridoma cell lines were prepared monoclonal antibodies against aflatoxin M1, an indirect competitive enzyme-linked immunosorbent assay detection method. The results can be developed a specific secreting monoclonal antibodies against aflatoxin M1 hybridoma cell lines, named 2F2. The monoclonal antibody Ig subclasses of IgG1, affinity constant of 28 * 10-11mol / L. The antibody and aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2 and aflatoxin M2 structure of analogues have weak cross-reaction with high specificity. On this basis, the establishment of an indirect competitive enzyme-linked immunosorbent assay detection method. The minimum detection limit of the method of 007ng/ml, the linear range of calibration curve 002 ~ 2ng/ml, linear equation y =- 04364x 02693 (R2 = 09949). Method of recovery was 725% ~ 1313%. Conclusions were prepared with high specificity and affinity of monoclonal antibodies against aflatoxin M1, and the establishment of a rapid, sensitive against aflatoxin M1 enzyme-linked immunosorbent assay detection method. Keywords:: aflatoxin M1; monoclonal antibody; enzyme-linked immunosorbent assay Development of monoclonal antibody against aflatoxin M1 and immunoassay for aflatoxin M1 Abstract: Objective To prepare monoclonal antibody against aflatoxin M1 and develop indirect competitive enzyme-linked immunosorbent assay for detection of aflatoxin M1.Methods Hybridoma cell line excreting monoclonal antibody against aflatoxin M1 was produced using B cell hybridoma technique and develop indi