Antisense bcl.docVIP

 PAGE \* MERGEFORMAT 6 Antisense bcl Author: Ge Zheng-Long, FAN Fang, Li Changfu, Lihai Xiang, Zeng Xiaoping [Keywords:] gene Effect of antisense oligodeoxynucleotide bcl2 on expression of Bcl2 protein and cell cycle of lens epithelial cells induced by galactose [Abstract] AIM: To investigate the effect of antisense oligodeoxynucleotide bcl2 (bcl2 ASODN) on the expression of Bcl2 protein and the cell cycle of rabbit lens epithelial cells (LECs) induced by galactose. METHODS: From translation initiation region of bcl2 gene, oligonucleotides were synthesized and modified with phosphorothioate. Rabbit lens were treated with galactose and bcl2 ASODN. The influence of bcl2 ASODN on the level of Bcl2 protein expression and the cell cycle of LECs induced by galactose was detected by flow cytometry. RESULTS: 5.0-15.0μ mol / L of bcl2 ASODN reduced the level of Bcl2 protein expression on LECs induced by galactose, with the fluorescence intensity decreasing from 158.70 ± 2.14 to 113.94 ± 2.88, and decreased the number of S phase cells of Lens from 20.60% to. 0.67%. CONCLUSION: bcl2 ASODN can inhibit the level of Bcl2 protein expression on LECs induced by galactose, interfere the entry of LECs into the S phase and inhibit the proliferation of LECs. [Keywords] genes, bcl2; oligonucleotides, antisense; galactose; lens, crystalline / cytology [Abstract] Objective: To investigate the bcl2 antisense oligonucleotide (bcl2 ASODN) on the rabbit lens induced by galactose in the Bcl2 protein expression and cell cycle. Methods: synthetic and bcl2 gene translation initiation sequence complementary oligonucleotide nucleotide and phosphorylation modification, with galactose and bcl2 ASODN processing in vitro cultured rabbit lens, flow cytometry bcl2 ASODN pairs of LEC induced by galactose in Bcl2 protein levels and cell cycle. Results: 5.0 ~ 15.0 μ mol / L antisense oligonucleotides can down-regulate bcl2 galactose-induced lens epithelial cells in the Bcl2 protein expression, f