Antisense oligonucleotide to the retinal pigment epithelial cells in the human telomerase reverse transcriptase expression and cell proliferation.docVIP

 PAGE \* MERGEFORMAT 9 Antisense oligonucleotide to the retinal pigment epithelial cells in the human telomerase reverse transcriptase expression and cell proliferation [Keywords:] retinal pigment epithelium;, Oligonucleotides, antisense;, telomerase reverse transcriptase Effect of antisense oligonucleotides on telomerase reverse transcriptase gene expression and retinal pigment epithelium cell proliferation [Abstract] AIM: To investigate the telomerase reverse transcriptase (TERT) gene expression in retinal pigment epithelium (RPE) cells and the inhibitory effect of antisense oligonucleotides (ASODN) encoding TERT mRNA to gene expression and proliferation of RPE cells, so as to search for a new genetic therapy for proliferative vitreoretinopathy (PVR). METHODS: (1) Rabbit RPE cells cultured in vitro were detected for TERT expression by streptavidinbiotin complex (SABC) immunohistochemistry at several time points. (2) The ASODN and sense oligodeoxynucleotides (SODN ) encoding TERT were delivered to the RPE cells at different concentrations, and then TERT expression was detected by immunohistochemistry. (3) Exposed to different concentrations of ASODN and SODN, proliferation activity and growthinhibitory rate of RPE cells were measured by MTT methods. RESULTS: TERT expression was markedly suppressed in the RPE cells treated with 5 and 10 μ mol / L TERT ASODN. TERT ASODN significantly inhibited proliferative activity of RPE cells in a dosedependent manner. CONCLUSION: ASODN complementary to TERT mRNA at some concentration can sequencespecifically suppress TERT expression in RPE cells and cellular proliferative activity, and show a potential application in further experimental study for PVR genetic medication. [Keywords] retinal pigment epithelium; oligonucleotides, antisense; telomerase reverse transcriptase [Abstract] Objective: To study the retinal pigment epithelium (RPE) cells, human telomerase reverse transcriptase (TERT) expression and its anti-sense o