Antitumor Effects of Total Glycoside Analysis.docVIP

 PAGE \* MERGEFORMAT 14 Antitumor Effects of Total Glycoside Analysis Study: CAO Li, Yang Weibin, Pan Rui Le, Li Lingling, Jin, Sun Hong, Sun Xiaobo [Abstract] Objective Total Glycoside of transplanted tumors in mice and human tumor cell xenografts in nude mice in vivo anti-tumor effects, as well as human tumor cell lines in vitro inhibitory activity and inhibition mechanism of its preliminary study . Methods MTT assay in vitro Total Glycoside on human tumor cell proliferation, observed in mice in vivo in nude mice transplanted S180 and A549 human lung cancer inhibition, while using flow cytometry and pathologic sections of the A549 tumor cell inoculation were observed. Results of Total Glycoside on the A549, HepG2, HL60, Eca-109, MDA-MB231 tumor cells, half inhibitory concentration (IC50) were 20.3,27.1,21.2,23.4 and 32.7 μg / mL. Total Glycoside of mice by oral 100 mg / kg and 200 mg / kg significantly inhibited the S180 tumor (tumor inhibition rates were 42.8% and 54.6%) and nude mice the growth of human lung adenocarcinoma A549 (T / C values #8203;#8203;were 58.1% and 52.2%), tumor biopsy and flow cytometry detection of apoptosis in A549 tumor cells showed cohosh can induce apoptosis of tumor cells in vivo. Conclusion Cohosh all have in vivo anti-tumor activity in vitro, in vivo inhibition of tumor growth may be associated with the induction of apoptosis. [Keywords: Total Glycoside, S180 sarcoma in mice, nude mice, human lung adenocarcinoma A549, apoptosis Keywords:: total glycoside of Cimicifuga dahurica, Sarcoma 180 tumor cells, nude mice, human pulmonary carcinoma A549, apoptosis Currently, cancer chemotherapy, considerable progress has been made, many cancers have been alleviated by chemical treatment or cure. However, there still exists Hualiao toxic side effects of drug and tumor cells to a large drug resistance problems, therefore, an urgent need to develop new efficient, low toxicity and antitumor drugs. cohosh has published Touzhen,