Application of magnetic separation enzyme-linked immunosorbent assay and clinical application of thyroid function.docVIP

 PAGE \* MERGEFORMAT 8 Application of magnetic separation enzyme-linked immunosorbent assay and clinical application of thyroid function [Abstract] Application of magnetic separation enzyme-linked immunosorbent assay determination of serum TSH, T3, T4, FT3, FT4 five indicators. Healthy group (n = 100), the establishment of the laboratory normal reference values. The hyperthyroidism who group (n = 68), hypothyroidism person group (n = 22), various acute or chronic systemic disease of the patients, of which 12 were myocardial infarction, congestive heart failure 8, hepatitis, liver cirrhosis 10, 11 cerebrovascular disease, respiratory infections 21. The results show that: healthy level of serum TSH is slightly skewed distribution, 2.5 to 97.5 percentile range (0.29 ~ 5.70) mIU / L, serum T3 levels were normal, X = 2.76, S = 1.02, normal reference values (1.74 ~ 3.78) mmol / L, serum T4 levels were normal X: 84.82, S = 26.23, normal reference range (58.59 ~ 111.05) mmol / L, serum FT3 levels were normal, X = 2.06, S = 0.88, normal reference range (1.18 ~ 2.94) pmoL / L, serum FT4 levels were normal X = 8.53, S = 3.03, normal reference range (5.5 ~ 11.56) pmol / L. TSH levels in patients with newly diagnosed hyperthyroidism, T3, T4, FT3, FT4 increased to varying degrees, in patients with hypothyroidism, elevated TSH, T3 , T4, FT3, FT4 decreased in varying degrees, a variety of acute or chronic systemic disease in patients with normal TSH levels and no significant difference, T3, FT3, FT4 normal or slightly lower, T4 normal or elevated, while the experimental observation of TSH , FT3, FT4 three indicators in the diagnosis of thyroid function on the sensitivity and specificity than T3, T4 indices. Magnetic Separation ELISA is a sensitive, specific, effective, and no radioactive contamination of the immunochemical detection. [Keywords:] gland of the original acid (T3) triiodothyronine four (T4) free three-triiodothyronine (FT3) triiodothyronine four free (